These mice express a transgene containing a fragment of human genomic DNA containing the S100B gene and its transcriptional control elements.
Dr. Alexander Marks, Univeristy of Toronto
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice homozygous for this transgene are viable and fertile. They have impaired learning and electrophysiological disturbances in the hippocampus. This strain may serve as a model of the mild learning impairment seen in human beings with Down syndrome (trisomy 21).
A transgene construct containing the human S100B gene was microinjected into fertilized CD1 embryos. The founder animal (H-5) contained approximately eight copies of the transgene.
Expressed Gene | S100B, S100 calcium binding protein B, human |
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Site of Expression |
Allele Name | transgene insertion 5, Alexander Marks |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | S100B TG; S-100beta; TghuS100B |
Gene Symbol and Name | Tg(huS100B)5Daoh, transgene insertion 5, Alexander Marks |
Gene Synonym(s) | |
Promoter | S100B, S100 calcium binding protein B, human |
Expressed Gene | S100B, S100 calcium binding protein B, human |
Strain of Origin | CD-1 |
Chromosome | UN |
General Note | Line 5 was derived from founder H-5, and carries 8 copies of the transgene. Other founders produced were H-2, H-8, and H-4, carrying 110, 70, and 10 copies of the transgene, respectively. The level of transgene expression was found to be directly correlated to the transgene copy number. Homozygous transgenic mice are viable and fertile. They have impaired learning and electrophysiological disturbances in the hippocampus. |
Molecular Note | A 17.3 kb fragment of human genomic DNA containing the S100B gene and its transcriptional control elements was used as the transgene. Expression of the human gene in transgenic animals was verified by Northern and Western blot analysis. Strong expression of the transgene was detected in the brain, and very low levels expression was detected in the liver, heart, and kidney. This line carries about 8 copies of the transgene. |
This strain was generated and is currently maintained on an outbred CD1 genetic background.
When using the STOCK Tg(huS100B)5Daoh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003198 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Assumed Homozygous for Tg(huS100B)5Daoh, (Tg/?) |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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