STOCK Tg(huS100B)5Daoh/J

Stock number: 003198
Research areas: Neurobiology Research, Research Tools, Sensorineural Research

Description

These mice express a transgene containing a fragment of human genomic DNA containing the S100B gene and its transcriptional control elements.

Detailed description

Mice homozygous for this transgene are viable and fertile. They have impaired learning and electrophysiological disturbances in the hippocampus. This strain may serve as a model of the mild learning impairment seen in human beings with Down syndrome (trisomy 21).

Development

A transgene construct containing the human S100B gene was microinjected into fertilized CD1 embryos. The founder animal (H-5) contained approximately eight copies of the transgene.

Allele

Symbol: Tg(huS100B)5Daoh
Name: transgene insertion 5, Alexander Marks
MGI accession ID: MGI:2386685
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(huS100B)5Daoh
Marker name: transgene insertion 5, Alexander Marks
Chromosome: UN
Strain of origin: CD-1
Expressed genes: S100B,S100 calcium binding protein B,human
Molecular note: A 17.3 kb fragment of human genomic DNA containing the S100B gene and its transcriptional control elements was used as the transgene. Expression of the human gene in transgenic animals was verified by Northern and Western blot analysis. Strong expression of the transgene was detected in the brain, and very low levels expression was detected in the liver, heart, and kidney. This line carries about 8 copies of the transgene.
General note: Line 5 was derived from founder H-5, and carries 8 copies of the transgene. Other founders produced were H-2, H-8, and H-4, carrying 110, 70, and 10 copies of the transgene, respectively. The level of transgene expression was found to be directly correlated to the transgene copy number.

Homozygous transgenic mice are viable and fertile. They have impaired learning and electrophysiological disturbances in the hippocampus.