| Allele Name | transgene insertion 5, Alexander Marks |
| Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
| Allele Synonym(s) | S100B TG; S-100beta; TghuS100B |
| Gene Symbol and Name | Tg(huS100B)5Daoh, transgene insertion 5, Alexander Marks |
| Gene Synonym(s) | |
| Promoter | S100B, S100 calcium binding protein B, human |
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| Expressed Gene | S100B, S100 calcium binding protein B, human |
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| Strain of Origin | CD-1 |
| Chromosome | UN |
| General Note | Line 5 was derived from founder H-5, and carries 8 copies of the transgene. Other founders produced were H-2, H-8, and H-4, carrying 110, 70, and 10 copies of the transgene, respectively. The level of transgene expression was found to be directly correlated to the transgene copy number. Homozygous transgenic mice are viable and fertile. They have impaired learning and electrophysiological disturbances in the hippocampus. |
| Molecular Note | A 17.3 kb fragment of human genomic DNA containing the S100B gene and its transcriptional control elements was used as the transgene. Expression of the human gene in transgenic animals was verified by Northern and Western blot analysis. Strong expression of the transgene was detected in the brain, and very low levels expression was detected in the liver, heart, and kidney. This line carries about 8 copies of the transgene. |
