JAX Mouse Strain Datasheet - 003184

B6.Cg-Myo7a^sh1-8J/J

Stock No:: 003184 |; shaker 1, 8 Jackson

Congenic

Cryo Recovery

Overview

Also Known As: shaker 1, 8 Jackson

These mice carry a spontaneous mutation at the Myo7a locus characterized by circling and head bobbing, similar to other Myo7a mutations.

Genetic overview

Genetic Background	Generation
000664 C57BL/6J

Myo7a^sh1-8J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Myo7a	myosin VIIA

View Genetics

Research Applications

Neurobiology Research
Sensorineural Research

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Myo7a^sh1-8J homozygous mice circle and bob their heads; heterozygotes are behaviorally normal. Homozygotes of both sexes are viable and fertile (Samples 2000). The hearing of Myo7a^sh1-8J mutants has not been examined. However, other Myo7a mutations, whose behavioral phenotypes are virtually identical to that of Myo7a^sh1-J, are associated with deafness.

Mutations in the human ortholog of Myo7a, MYO7A, have been characterized in pedigrees of patients having Usher syndrome type 1 (USH1), an autosomal recessive disorder characterized by profound deafness, vestibular dysfunction and progressive retinitis pigmentosa (Weil et al., 1995; Weston et al., 1996; Adato et al., 1997; Levy et al., 1997; Liu et al., 1998; Espinos et al., 1998; Cuevas et al., 1998, 1999; Janecke et al., 1999). MYO7A mutations have also been identified in patients with autosomal dominant non-syndromic deafness (Liu et al., 1997) and autosomal recessive isolated deafness (Liu et al., 1997; Weil et al., 1997).

Development

The Myo7a^sh1-8J mutation occurred spontaneously on B6,SJL-TgN(c177lacZ)226Bri while the latter was being bred in the Importation facility at The Jackson Laboratory. Myo7a^sh1-8J was transferred to the C57BL/6J background by seven rounds of backcross-intercross breeding; homozygous males produced by mating heterozygous sibs were bred to C57BL/6J females to yield the next generation of heterozygotes. The colony was genotyped for TgN(c177lacZ)226Bri in March, 2000 and verified free of the transgene.

Control Suggestions

Heterozygote from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Myo7a^sh1-8J

Allele Symbol: Myo7a^sh1-8J

Allele Name	shaker 1, 8 Jackson
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Myo7a, myosin VIIA
Gene Synonym(s)	DFNA11; DFNB2; Hdb; Hdb; MYOVIIA; MYU7A; Myo7; Myo7; NSRD2; USH1B; headbanger; myosin VII; neuroscience mutagenesis facility, 371; nmf371; nmf371; polka; polka; polka; sh-1; sh1; shaker 1
Strain of Origin	B6;SJL-Tg(c177-lacZ)226Bri/J
Chromosome	7
General Note	Genetic Background: The 266Bri stock on which Myo7a^sh1-8J arose was homozygous for a lacZ transgene. The mutation was not the result of the transgene integration, as not all animals exhibited the neurological phenotype.
Molecular Note	Two genomic regions encompassing a total of about 6.4 kb was deleted n this mutation. One region spans about 3.6 kb and covers exons 38 through 40. The other region spans about 2.8 kb and covers exons 46 and 47. Immunoblot analysis failed to detect protein in extracts from homozygous mice.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Myo7a^sh1-8J related

Neurobiology Research
- Hearing Defects
- Tremor Defects
- Vestibular Defects
Sensorineural Research
- Hearing Defects
- Vestibular Defects

Mammalian Phenotype Terms by Genotype

Genotype: Myo7a^sh1-8J/Myo7a⁺
B6.Cg-Myo7a^sh1-8J/J

hearing/vestibular/ear phenotype

impaired hearing
- slight but significant impairment compared to C57BL/6J controls only in older mice

Genotype: Myo7a^sh1-8J/Myo7a^sh1-8J
B6.Cg-Myo7a^sh1-8J/J

hearing/vestibular/ear phenotype

abnormal cochlear hair cell stereociliary bundle morphology
- hair bundles are disorganized at P2 and P6

abnormal vestibular hair cell stereociliary bundle morphology
- hair bundles are disorganized at P2 and P6

increased or absent threshold for auditory brainstem response
- lack ABR response even at the highest stimulus intensity (100 dB sound pressure level)

behavior/neurological phenotype

abnormal parental behavior
- homozygotes of both sexes breed, but are not good parents

circling
- mice walk in a circling motion

head bobbing

pigmentation phenotype

pigmentation phenotype
- mice exhibit wild-type iris pigmentation

nervous system phenotype

abnormal cochlear hair cell stereociliary bundle morphology
- hair bundles are disorganized at P2 and P6

abnormal vestibular hair cell stereociliary bundle morphology
- hair bundles are disorganized at P2 and P6

References

Additional References

Gibson F; Walsh J; Mburu P; Varela A; Brown KA; Antonio M; Beisel KW; Steel KP; Brown SD. 1995. A type VII myosin encoded by the mouse deafness gene shaker-1. Nature 374(6517):62-4. PubMed: 7870172 MGI: J:23257
Weil D; Blanchard S; Kaplan J; Guilford P; Gibson F; Walsh J; Mburu P; Varela A; Levilliers J; Weston MD; Kelley PM; Kimberling WJ; Wagenaar M; Levi-Acobas F; Larget-Piet D; Munnich A; Steel KP; Brown SDM; Petit C. 1995. Defective myosin VIIA gene responsible for Usher syndrome type 1B. Nature 374(6517):60-1. PubMed: 7870171 MGI: J:23239
Weil D; Kussel P; Blanchard S; Levy G; Levi-Acobas F; Drira M ; Ayadi H ; Petit C. 1997. The autosomal recessive isolated deafness, DFNB2, and the Usher 1B syndrome are allelic defects of the myosin-VIIA gene. Nat Genet 16(2):191-3. PubMed: 9171833 MGI: J:40770

Additional - Myo7a^sh1-8J related

Anderson MG; Hawes NL; Trantow CM; Chang B; John SW. 2008. Iris phenotypes and pigment dispersion caused by genes influencing pigmentation. Pigment Cell Melanoma Res 21(5):565-78. PubMed: 18715234 MGI: J:141035
Morgan CP; Krey JF; Grati M; Zhao B; Fallen S; Kannan-Sundhari A; Liu XZ; Choi D; Muller U; Barr-Gillespie PG. 2016. PDZD7-MYO7A complex identified in enriched stereocilia membranes. Elife 5:. PubMed: 27525485 MGI: J:236403
Osterweil E; Wells DG; Mooseker MS. 2005. A role for myosin VI in postsynaptic structure and glutamate receptor endocytosis. J Cell Biol 168(2):329-38. PubMed: 15657400 MGI: J:95877
Samples RM; Ward-Bailey P; Washburn LL; Donahue LR. 2002. A new shaker 1 mutation of the Myo7 gene in the mouse MGI Direct Data Submission :. MGI: J:77937
Zheng QY; Scarborough JD; Zheng Y; Yu H; Choi D; Gillespie PG. 2012. Digenic inheritance of deafness caused by 8J allele of myosin-VIIA and mutations in other Usher I genes. Hum Mol Genet 21(11):2588-98. PubMed: 22381527 MGI: J:183898

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Homozygous for Myo7a<sh1-8J>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: shaker 1, 8 Jackson

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Myo7ash1-8J

Allele Symbol: Myo7ash1-8J

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Myo7ash1-8J related

Mammalian Phenotype Terms by Genotype

Genotype: Myo7ash1-8J/Myo7a+B6.Cg-Myo7ash1-8J/J

hearing/vestibular/ear phenotype

Genotype: Myo7ash1-8J/Myo7ash1-8JB6.Cg-Myo7ash1-8J/J

hearing/vestibular/ear phenotype

behavior/neurological phenotype

pigmentation phenotype

nervous system phenotype

References

Additional References

Additional - Myo7ash1-8J related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Myo7a^sh1-8J

Allele Symbol: Myo7a^sh1-8J

Genotype: Myo7a^sh1-8J related

Genotype: Myo7a^sh1-8J/Myo7a⁺
B6.Cg-Myo7a^sh1-8J/J

Genotype: Myo7a^sh1-8J/Myo7a^sh1-8J
B6.Cg-Myo7a^sh1-8J/J

Additional - Myo7a^sh1-8J related