These Cdh2 knock-out mice exhibit embryonic lethality and malformation of structures resulting from neurulation and somitogenesis.
Dr. Richard Hynes, Massachusetts Institute of Technology
Mice that are heterozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. However, homozygous mice have an embryonic lethal phenotype, and die by embryonic day 10. The somites of the mutant embryos are small, irregularly shaped, and less cohesive compared with those of their wild-type littermates, and the epithelial organization of the somites is partially disrupted. Undulation of the neural tube is also observed in the mutant embryos. The mesodermal and endodermal cell layers of the yolk sac are separated in the mutants. The most dramatic cell adhesion defect is observed in the primitive heart; although myocardial tissue forms initially, the myocytes subsequently dissociate and the heart tube fails to develop normally.
A BamHI-BamHI 8.3 kb fragment was isolated from lambda clone, MNG16. The BamHI genomic DNA fragment was subcloned into a plasmid containing the PMC1-HSVTK cassette. The neo expression cassette, PGKneobpA, was digested with XhoI/SalI, and subcloned into a unique SalI site in exon 10. The construct was linearized with NotI and electroporated into 129S2/SvPas-derived D3 ES cells, and colonies were selected with G418 and gancyclovir. A 2.4 kb SmaI-HindIII genomic DNA fragment located immediately 3' to the short arm of the targeting vector was used as a probe. The targeted ES clones were injected into blastocysts from C57BL/6J mice.
|Allele Name||targeted mutation 1, Richard Hynes|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Cdh2tm1Hyn; targeted mutation 1, Richard Hynes|
|Gene Symbol and Name||Cdh2, cadherin 2|
|Gene Synonym(s)||cadherin, N-; NCAD; CD325; N-cadherin; Ncad; CDw325; CDHN; Ncad; N-CAD|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin cassette was inserted into the exon 10, which encodes part of the extracellular domain of the protein. The protein was untectectable in Western blots of protein lysates derived from homozygous mutant embryos.|
|Mutations Made By|| |
Dr. Richard Hynes, Massachusetts Institute of Technology
The strain exists on a mixed B6,129,CD1 background and can be maintained by heterozygous matings.
When using the N-cadherin KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003179 in your Materials and Methods section.
|Heterozygous or Wild-type for Cdh2<tm1Hyn>|
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