These Arnt knock-out mice exhibit failure in response to hypoxia and to hypoglycaemia. They are suitable for use in applications related to the study of angiogenesis and vasoformation during mammalian embryonic development.
M. Celeste Simon, University of Pennsylvania
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Arnt | aryl hydrocarbon receptor nuclear translocator |
Arnt-/- embryonic stem cells fail to activate genes that normally respond to low oxygen tension. They also fail to respond to a decrease in glucose concentration, indicating that ARNT is crucial in the response to hypoxia and to hypoglycaemia. Arnt-/- embryos were not viable past embryonic day 10.5 and showed defective angiogenesis of the yolk sac and branchial arches, stunted development and embryo wasting. The defect in blood vessel formation in Arnt-/- yolk sacs is similar to the angiogenic abnormalities reported for mice deficient in vascular endothelial growth factor or tissue factor.
The exon encoding the basic-helix-loop-helis (bHLH) domain of the murine Arnt gene was disrupted by insertion of the phosphoglycerate kinase (PGK) promoter/neomycin-resistance cDNA.
Allele Name | targeted mutation 1, M Celeste Simon |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Arnt-; Arnt(-) |
Gene Symbol and Name | Arnt, aryl hydrocarbon receptor nuclear translocator |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 3 |
Molecular Note | A PGK-neomycin resistance cassette was inserted into the exon that encodes the basic-helix-loop-helix domain. |
Mutations Made By | M. Celeste Simon, University of Pennsylvania |
Homozygous mutants are not viable past embryonic day 10.5. The expected coat color is black.
When using the Arnt- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003178 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Arnt<tm1Mcs> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.