There is complete female sterility due to abberant estrous cycles, abnormal preimplantation development of eggs, no implantation of blastocysts, lack of pseudopregnancy. Males show slightly delayed fertility. Mammary development is markedly affected. Homozygotes have no mammary development and do not lactate. Heterozygotes are unable to lactate after the first pregnancies, but attain some degree of lactation as they age or after multiple pregnancies. Serum prolactin levels are increased 60 - 100 fold in both males and females. Maternal behavior is diminished in pimiparous and nulliparous animals. Bone remodelling is decreased in homozygote mutants.
Exon 5 was replaced with the neomycin resistance gene driven by the thymidine kinase promotor. The truncated mRNA, if produced, would encode a protein of only 44 amino acids, unable to bind the hormone and produce any biological action. The targeting construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to crossed to C57BL/6 and offspring were backcrossed to C57BL/6 for at least 10 generations.
|Allele Name||targeted mutation 1, Paul A Kelly|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Prlr-; PRLRko|
|Gene Symbol and Name||Prlr, prolactin receptor|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A neomycin cassette was inserted into exon 5, creating an in-frame stop codon. Northern, Western, and binding data all demonstrate the lack of a functional receptor in homozygous mutant animals.|
|Mutations Made By|| |
Dr. Paul Kelly, Faculte de Medecine Necker
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony. Homozygous females are sterile and males exhibit delayed fertility.
When using the PRLR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003142 in your Materials and Methods section.