These mice harbor the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP); PB-Tag Line 8247 transgene. Mice hemizygous for the TRAMP transgene develop progressive forms of prostate cancer with distant site metastasis and exhibit various forms of disease from mild intraepithelial hyperplasia to large multinodular malignant neoplasia.
Dr. Norman M. Greenberg, Pfizer Global Research and Development
Genetic Background | Generation |
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N?+12pN32
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Starting at:
$255.00 Domestic price for female 4-week |
281.22 Domestic price for breeder pair |
These mice harbor the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP); PB-Tag Line 8247 transgene. Mice hemizygous for the TRAMP transgene develop progressive forms of prostate cancer with distant site metastasis and exhibit various forms of disease from mild intraepithelial hyperplasia to large multinodular malignant neoplasia. TRAMP hemizygotes can exhibit prostatic epithelial neoplasia (PIN) by 12 weeks of age. Tumors, appearing as well differentiated adenocarcinoma, can arise by 24 weeks of age, mostly in the dorsal and lateral lobes of the prostate. By 30 weeks of age, most hemizygous mice will display evidence of metastatic spread to the lymph nodes and/or lungs, phylloides appearance of some tumors, and seminal vesicle invasion. Tumors exhibit elevated levels of nuclear TRP53 and decreased androgen receptor expression. Note that pure C57BL/6 TRAMP hemizygotes may live to 52 weeks of age or longer.
These TRAMP transgenic mice were developed in the laboratory of Dr. Norman M. Greenberg at Baylor College of Medicine. The TRAMP (Transgenic Adenocarcinoma of Mouse Prostate) transgenic construct was designed with simian virus 40 (SV40) early genes (large and small tumor antigens, Tag) under the control of the rat probasin promoter. The founder mouse (line 8247) described in the primary publication is one that developed a large prostate tumor by 10 weeks of age and was generated on a C57BL/6 background. The donating investigator maintains the strain by breeding hemizygous females with C57BL/6 males. The strain has been backcrossed to C57BL/6 for 20+ generations.
In 2019, sequencing performed by The Jackson Laboratory discovered that greater than 20 copies of the Tg(TRAMP)8247Ng transgene (hemizygous) integrated on chromosome 16 (~18,993,000-19,272,880; mouse mm10). The region surrounding the integration site has duplicated. Currently, there are no genes annotated in this region.
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
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Site of Expression |
Allele Name | transgene insertion 8247, Norman M Greenberg |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | PB-Tag; probasin/Tag; Tg(Pbsn-TAg)8247Ng; TRAMP |
Gene Symbol and Name | Tg(TRAMP)8247Ng, transgene insertion 8247, Norman M Greenberg |
Gene Synonym(s) | |
Promoter | Pb, probasin, rat |
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
Strain of Origin | C57BL/6 |
Chromosome | 16 |
General Note | TRAMP stands for TRansgenic Adenocarcinoma Mouse Prostate. Lines 5666 and 8079 were also produced. Transgenic mice on a C57BL6 background develop progressive forms of prostate cancer with distant site metastasis. Forms of disease range from mild intraepithelial hyperplasia to large multinodular malignant neoplasia. Tumors are detected in the prostate as early as 10 weeks of age. The tumors have elevated levels of nuclear TRP53 and decreased androgen receptor expression. |
Molecular Note | Expression of the SV40 early region tumor antigen was driven in mouse prostate epithelium via a rat probasin promoter region fragment (-426/+28 bp). Sequencing identified greater than 20 copies of the Tg(TRAMP)8247Ng transgene (hemizygous) integrated on chromosome 16 (~18,993,000-19,272,880; mouse mm10). The region surrounding the integration site has duplicated. Currently, there are no genes annotated in this region. |
Mutations Made By | Dr. Norman Greenberg, Pfizer Global Research and Development |
When maintaining our live colony, C57BL/6J inbred mice are bred to hemizygous mice. Most commonly, but not exclusively, this is C57BL/6J females bred to hemizygous males. Expected coat color from breeding is black. [2020]
When using the B6 TRAMP mice mouse strain in a publication, please cite the originating article(s) and include JAX stock #003135 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(TRAMP)8247Ng |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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