This strain has been discontinued. The Jackson Laboratory Repository has been provided with sufficient evidence that the mutation is not targeted to the Bcl3 gene in the manner described in the primary publication (Schwarz et al. 1997 Genes Dev 11:187). While the exact targeting event is in question, published data demonstrate the lack of functional BCL3 protein in this mutant stock (Hunter and Kandarian 2004 J Clin Invest 114:1504). Should new data describing the targeting event in Stock No. 003127 become available, we will update our strain data sheet and reconsider the availability of this stock. Please search the JAX® Mice Database for an alternative strain.
Dr. Anton Berns, University of Amsterdam
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Bcl3 | B cell leukemia/lymphoma 3 |
Allele Name | targeted mutation 1, Inder M Verma |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Bcl3(-); Bcl3tm1 |
Gene Symbol and Name | Bcl3, B cell leukemia/lymphoma 3 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
General Note | The Jax strain JR003127 strain has been discontinued. The Jackson Laboratory Repository has been provided with sufficient evidence that the mutation is not targeted to the Bcl3 gene in the manner described in the primary publication (Schwarz et al. 1997 Genes Dev 11:187). While the exact targeting event is in question, published data demonstrate the lack of functional BCL3 protein in this mutant stock (Hunter and Kandarian 2004 J Clin Invest 114:1504). |
Molecular Note | A ~10 kb genomic fragment containing exons 1-6 was replaced with a hygromycin selection cassette inserted in reverse transcriptional orientation. These sequences encode five ankyrin repeats of the protein. Western blot analysis on spleen lysates derived from homozygous mice confirmed that no detectable protein was produced from this allele. |
Mutations Made By | Dr. Anton Berns, University of Amsterdam |
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