FVB;129P2-Bcl3^tm1Ver/J

Stock number: 003127
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

This strain has been discontinued. The Jackson Laboratory Repository has been provided with sufficient evidence that the mutation is not targeted to the Bcl3 gene in the manner described in the primary publication (Schwarz et al. 1997 Genes Dev 11:187). While the exact targeting event is in question, published data demonstrate the lack of functional BCL3 protein in this mutant stock (Hunter and Kandarian 2004 J Clin Invest 114:1504). Should new data describing the targeting event in Stock No. 003127 become available, we will update our strain data sheet and reconsider the availability of this stock. Please search the JAX^® Mice Database for an alternative strain.

Allele

Symbol: Bcl3^tm1Ver
Name: targeted mutation 1, Inder M Verma
MGI accession ID: MGI:1861796
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Bcl3
Marker name: B cell leukemia/lymphoma 3
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Molecular note: A ~10 kb genomic fragment containing exons 1-6 was replaced with a hygromycin selection cassette inserted in reverse transcriptional orientation. These sequences encode five ankyrin repeats of the protein. Western blot analysis on spleen lysates derived from homozygous mice confirmed that no detectable protein was produced from this allele.
General note: The Jax strain JR003127 strain has been discontinued. The Jackson Laboratory Repository has been provided with sufficient evidence that the mutation is not targeted to the Bcl3 gene in the manner described in the primary publication (Schwarz et al. 1997 Genes Dev 11:187). While the exact targeting event is in question, published data demonstrate the lack of functional BCL3 protein in this mutant stock (Hunter and Kandarian 2004 J Clin Invest 114:1504).