B6.129X1-Grpr^tm1Jfb/J

Stock number: 003126
Product name: GRP-R KO
Research areas: Cancer Research, Diabetes and Obesity Research, Endocrine Deficiency Research, Neurobiology Research

Description

These Grpr knock-out mice exhibit elevation of body weight in older animals associated with a deficiency in bombesin-induced mediation of satiety.

Detailed description

Mice homozygous (females) or hemizygous (males) for the X-linked Grpr^tm1Jfb targeted mutation are viable with no gross phenotypic abnormalities observed. There is a deficiency in bombesin-induced mediation of satiety as measured by glucose intake. A modest increase in body weight is observed in older animals. At 18-20 months of age, hemizygous males (N = 8) are 44.5g ± 5.1 (mean ± std dev) and wildtype littermates (N = 8) are 38.3g ± 5.1 (L. Hampton, unpublished observation). We have recently observed head tilts and bobbing in the hemizygous males in our production colony. The donating investigator of this strain has also observed this phenotype and considers it a mutation not related to the gene. We are working to remove this phenotype, but we will continue to ship from the colony while we finish this process.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for 8 generations.

Allele

Symbol: Grpr^tm1Jfb
Name: targeted mutation 1, James F Battey
MGI accession ID: MGI:1857462
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Grpr
Marker name: gastrin releasing peptide receptor
Chromosome: X
Strain of origin: 129X1/SvJ
Molecular note: A neomycin resistance cassette disrupted exon 1 of the gene.