These Grpr knock-out mice exhibit elevation of body weight in older animals associated with a deficiency in bombesin-induced mediation of satiety.
Dr. Lori Hampton, NIDCD-NIH
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Grpr | gastrin releasing peptide receptor |
Mice homozygous (females) or hemizygous (males) for the X-linked Grprtm1Jfb targeted mutation are viable with no gross phenotypic abnormalities observed. There is a deficiency in bombesin-induced mediation of satiety as measured by glucose intake. A modest increase in body weight is observed in older animals. At 18-20 months of age, hemizygous males (N = 8) are 44.5g ± 5.1 (mean ± std dev) and wildtype littermates (N = 8) are 38.3g ± 5.1 (L. Hampton, unpublished observation). We have recently observed head tilts and bobbing in the hemizygous males in our production colony. The donating investigator of this strain has also observed this phenotype and considers it a mutation not related to the gene. We are working to remove this phenotype, but we will continue to ship from the colony while we finish this process.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for 8 generations.
Allele Name | targeted mutation 1, James F Battey |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GRP-R-; GRPR- |
Gene Symbol and Name | Grpr, gastrin releasing peptide receptor |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | X |
Molecular Note | A neomycin resistance cassette disrupted exon 1 of the gene. |
Mutations Made By | Dr. Lori Hampton, NIDCD-NIH |
When maintaining a live colony, these mice are bred by crossing heterozygous or homozygous females and hemizygous males. This is a targeted mutation of an X-linked gene. Expected coat color from breeding: Black.
When using the GRP-R KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003126 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous or Wild-type for Grpr<tm1Jfb> |
Frozen Mouse Embryo | B6.129X1-Grpr<tm1Jfb>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Grpr<tm1Jfb>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Grpr<tm1Jfb>/J | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Grpr<tm1Jfb>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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