B6;129-Grm5^tm1Rod/J

Stock number: 003121
Product name: mGluR5-
Research areas: Neurobiology Research

Description

These Grm5 knock-out mice exhibit a reduction in long-term potentiation in glutamate receptor and impairments in the acquisition and use of spatial information.

Detailed description

Mice homozygous for the targeted mutation are viable and fertile. Long-term potentiation (LTP) in homozygous mutant mice is significantly reduced in the NMDA receptor (NMDAR)-dependent pathways such as the CA1 region and dentate gyrus of the hippocampus, whereas LTP remains intact in the mossy fiber synapses on the CA3 region, an NMDAR-independent pathway. These mutant mice also show impairments in the acquisition and use of spatial information in both the Morris water maze and contextual information in the fear-conditioning test.

Development

The mutant allele was made by replacing the first exon of the Grm5 (mGluR5) (G protein coupled receptor, family C, group 1, member E) locus with a neo marker. The mice were sent to The Jackson Laboratory on a B6;129 background background.

Allele

Symbol: Grm5^tm1Rod
Name: targeted mutation 1, John C Roder
MGI accession ID: MGI:1857437
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Grm5
Marker name: glutamate receptor, metabotropic 5
Chromosome: 7
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was designed to delete a 0.4 kb fragment containing part of exon 1 and a part of intron 1, with a neomycin resistance cassette. Western blot failed to detect protein in mutants.