These Grm5 knock-out mice exhibit a reduction in long-term potentiation in glutamate receptor and impairments in the acquisition and use of spatial information.
Dr. John Roder, University of Toronto
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Grm5 | glutamate receptor, metabotropic 5 |
Mice homozygous for the targeted mutation are viable and fertile. Long-term potentiation (LTP) in homozygous mutant mice is significantly reduced in the NMDA receptor (NMDAR)-dependent pathways such as the CA1 region and dentate gyrus of the hippocampus, whereas LTP remains intact in the mossy fiber synapses on the CA3 region, an NMDAR-independent pathway. These mutant mice also show impairments in the acquisition and use of spatial information in both the Morris water maze and contextual information in the fear-conditioning test.
The mutant allele was made by replacing the first exon of the Grm5 (mGluR5) (G protein coupled receptor, family C, group 1, member E) locus with a neo marker. The mice were sent to The Jackson Laboratory on a B6;129 background background.
Allele Name | targeted mutation 1, John C Roder |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Gprc1etm1Rod; mGluR5- |
Gene Symbol and Name | Grm5, glutamate receptor, metabotropic 5 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | A targeting vector was designed to delete a 0.4 kb fragment containing part of exon 1 and a part of intron 1, with a neomycin resistance cassette. Western blot failed to detect protein in mutants. |
Mutations Made By | Dr. John Roder, University of Toronto |
Homozygotes are viable and fertile. When maintaining a live colony, heterozygous or homozygous mice may be bred together.
When using the mGluR5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003121 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Grm5<tm1Rod> |
Frozen Mouse Embryo | B6;129-Grm5<tm1Rod>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Grm5<tm1Rod>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Grm5<tm1Rod>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129-Grm5<tm1Rod>/J Frozen Embryos | $3373.50 |
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