The L1 gene is localized to the X chromosome. As a result, hemizygous males are affected and the mutation has to be propagated through females. Male mice hemizygous for the L1camtm1Sor targeted mutation have defects in the guidance of axons of the corticospinal tract. A substantial proportion of axons do not take their normal crossed course to the dorsal column at the pyramidal decussation. There is also a varying, but reduced number of corticospinal axons in the dorsal columns of the spinal cord.
|Allele Name||targeted mutation 1, Philippe Soriano|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||L1cam, L1 cell adhesion molecule|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||A neomycin resistance cassette replaced the exons encoding the sixth immunoglobulin-like domain of the protein. Immunoblots of lysates of adult brains from homozygous mutant mice showed an absence of normal protein.|
|Mutations Made By|| |
Dr. Philippe Soriano, Mount Sinai School of Medicine
L1 is X-linked. When maintaining a live colony, heterozygous females may be bred to males from the colony.
When using the L1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003120 in your Materials and Methods section.