Two-thirds of homozygous Sp4tm1Ssp mutants die within the first few days after birth and those that survive are smaller than their wild-type littermates. While fertility of the female mutants appears normal, homozygous mutant males do not breed, despite having histologically intact testes containing mature sperm. Homozygous Sp4tm1Ssp mutant males fail to copulate, indicating that this gene is required for normal male reproductive behavior.
|Allele Name||targeted mutation 1, S Steven Potter|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Sp4, trans-acting transcription factor 4|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Sequence encoding three zinc-finger domains was replaced with a construct containing an HPRT gene. Northern blot analysis of brain extracts showed the presence of an aberrant transcript in homozygous mutant mice. The transcript was undetectable in further analysis using a probe specific for the zinc-finger domains.|
|Mutations Made By|| |
Dr. Jennifer Lachey, The Children's Hospital
When maintaining a live colony, heterozygous mice may be bred together.
When using the Sp4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003119 in your Materials and Methods section.