STOCK Sp4^tm1Ssp/J

Stock number: 003119
Product name: Sp4 KO
Research areas: Cell Biology Research, Developmental Biology Research, Neurobiology Research, Reproductive Biology Research

Description

These Sp4 knock-out mice exhibit early death and failure of males to breed.

Detailed description

Two-thirds of homozygous Sp4^tm1Ssp mutants die within the first few days after birth and those that survive are smaller than their wild-type littermates. While fertility of the female mutants appears normal, homozygous mutant males do not breed, despite having histologically intact testes containing mature sperm. Homozygous Sp4^tm1Ssp mutant males fail to copulate, indicating that this gene is required for normal male reproductive behavior.

Allele

Symbol: Sp4^tm1Ssp
Name: targeted mutation 1, S Steven Potter
MGI accession ID: MGI:1857451
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sp4
Marker name: trans-acting transcription factor 4
Chromosome: 12
Strain of origin: 129P2/OlaHsd
Molecular note: Sequence encoding three zinc-finger domains was replaced with a construct containing an HPRT gene. Northern blot analysis of brain extracts showed the presence of an aberrant transcript in homozygous mutant mice. The transcript was undetectable in further analysis using a probe specific for the zinc-finger domains.