These Crh knock-out mice exhibit reduced adrenocortical secretion following stress. They are suitable for use in applications related to the study of stress response and fetal development.
IMR Colony, The Jackson Laboratory
Mice homozygous for the Crhtm1Maj targeted mutation are viable and fertile. Pups born to homozygous mothers must be supplemented with corticosterone in the drinking water from day 12 of gestation until weaning. Homozygous mice have reduced adrenocortical secretion following stress. They may be susceptible to complications from hypoglycemia when fasting. Heterozygous mice are phenotypically indistinguishable from normal wildtype siblings.
This strain was developed in the laboratory of Dr. Joseph Majzoub at Children's Hospital, Boston. The vector containing Pgk-neo replaced the entire coding region of the endogenous gene. The 129S2/SvPas-derived D3 ES cell line was used. The strain was backcrossed to C57BL/6J in the IMR colony.
|Allele Name||targeted mutation 1, Joseph Majzoub|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CRH -; CRH KO; CRHKO; CRH-KO|
|Gene Symbol and Name||Crh, corticotropin releasing hormone|
|Strain of Origin||129S2/SvPas|
|Molecular Note||The sequences encoding the pre-propeptide region were replaced by a neomycin resistance cassette. RT-PCR analysis demonstrated that the transcript was absent in whole brain RNA derived from homozygous mice. Immunohistochemical analysis demonstrated that the protein was not detectable in sections of the amygdala and cerebral cortex of brains of homozygous mice.|
|Mutations Made By|| |
Dr. Joseph Majzoub, Children's Hospital Boston
When maintaining a live colony, these mice are maintained by mating homozygous siblings accompanied by maternal corticosterone treatment. Alternatively, heterozygous females can be bred to homozygous males; this strategy requires no corticosterone treatment.
When using the CRH KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003114 in your Materials and Methods section.