STOCK Itgb1^tm1Lscd/J

Stock number: 003096
Product name: Β1 KO
Research areas: Developmental Biology Research

Description

These Itgb1 knock-out mice die during early post-implantation development. They are suitable for use in studies of morphogenesis and gene regulation during embryonic development.

Detailed description

Mice homozygous for the Itgb1^tmlLscd targeted mutation die during early postimplantation development. Beta1-null embryos form normal-looking blastocysts and initiate implantation at E4.5. By E5.5, Beta1-null embryos degenerate extensively. The inner cell mass region of blastocyst outgrowths show highly retarded growth and defective extra-embryonic endoderm morphogenesis and migration.

Development

Exon 2 of this integrin beta 1 allele was disrupted using the targeting vector pBSb1/neo/tk. The vector contains 6 kb of the 5' region, spanning exons 2-4, the first three coding exons, and both neomycin (promotorless) and herpes simplex virus thymidine kinase (HSC-tk) cassette to allow for a positive/negative selection scheme. The construct was electroporated into 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J and offspring were bred to black Swiss Webster stock.

Allele

Symbol: Itgb1^tm1Lscd
Name: targeted mutation 1, Caroline H Damsky
MGI accession ID: MGI:2154540
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: beta1-null; Itgbeta1^-
Marker symbol: Itgb1
Marker name: integrin beta 1 (fibronectin receptor beta)
Marker synonyms: 4633401G24Rik; beta1 integrin; CD29; FNRB; Gm9863; GPIIA; MDF2; MSK12; VLAB; VLA-BETA
Chromosome: 8
Strain of origin: 129X1/SvJ
Molecular note: A promoterless neomycin resistance gene fusion was inserted in-frame into exon 2 of the gene.