These Itgb1 knock-out mice die during early post-implantation development. They are suitable for use in studies of morphogenesis and gene regulation during embryonic development.
Dr. Caroline Damsky, Univ of California, San Francisco
Mice homozygous for the Itgb1tmlLscd targeted mutation die during early postimplantation development. Beta1-null embryos form normal-looking blastocysts and initiate implantation at E4.5. By E5.5, Beta1-null embryos degenerate extensively. The inner cell mass region of blastocyst outgrowths show highly retarded growth and defective extra-embryonic endoderm morphogenesis and migration.
Exon 2 of this integrin beta 1 allele was disrupted using the targeting vector pBSb1/neo/tk. The vector contains 6 kb of the 5' region, spanning exons 2-4, the first three coding exons, and both neomycin (promotorless) and herpes simplex virus thymidine kinase (HSC-tk) cassette to allow for a positive/negative selection scheme. The construct was electroporated into 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J and offspring were bred to black Swiss Webster stock.
|Allele Name||targeted mutation 1, Caroline H Damsky|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||beta1-null; Itgbeta1-|
|Gene Symbol and Name||Itgb1, integrin beta 1 (fibronectin receptor beta)|
|Strain of Origin||129X1/SvJ|
|Molecular Note||A promoterless neomycin resistance gene fusion was inserted in-frame into exon 2 of the gene.|
|Mutations Made By|| |
Dr. Laurie Stephens, Univ of California, San Francisco
When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. Mice homozygous for the mutation are not viable.
When using the Β1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003096 in your Materials and Methods section.