These mice carry a spontaneous mutation at the Vac14 locus characterized by small size and prevalent hydrocephalus. They are suitable for use in applications related to the study of the phosphoinositide phosphate regulatory complex and neuron development in the mammalian nervous system.Read More +
Mice homozygous for the recessive Vac14ingls mutation are significantly smaller than their littermates, and their coat color is dilute with white underfur both dorsally and ventrally (Sweet 1991; Samples 2003). Most develop hydrocephalus with onset by one week of age. Homozygotes are weak and uncoordinated and have difficulty righting themselves. They fail to gain weight during the second week of life, and most die by three weeks of age. (Bronson et al. 2003) Length of survival appears to correlate with the severity of hydrocephalus, which varies from absent to severe; however, all homozygotes die by four weeks, whether or not they are hydrocephalic (Samples et al. 2003).
Hydrocephalus affects the lateral ventricles most severely; serial sections reveal an intact cerebral aqueduct. Diffuse astrocytic hypertrophy and hyperplasia are observed in brains and spinal cords of mutant mice. A few mutants develop status spongiosis, and a few exhibit gliosis alone. Astrocytes cultured from affected mice stop dividing within three weeks, but survive for up to 12 weeks. They do not develop into tumors when implanted into the brains of normal mice. (Bronson et al. 2003)
ingls arose spontaneously in a breeding colony of the inbred strain DBA/2J at The Jackson Laboratory in 1991. It has been backcrossed onto C57BL/6J and is maintained by crossing hosts of homozygous ovarian transplants to C57BL/6J males, then intercrossing the resultant obligate heterozygotes. In October 2003 this strain had reached generation N19F1.
|Allele Name||infantile gliosis|
|Allele Type||Spontaneous (Hypomorph)|
|Allele Synonym(s)||infantile gliosis; Vac14ingls|
|Gene Symbol and Name||Vac14, Vac14 homolog (S. cerevisiae)|
|Gene Synonym(s)||ArPIKfyve; D8Wsu151e; ingls; DNA segment, Chr 8, Wayne State University 151, expressed; expressed sequence AA959718; Tax1bp2; TRX; MGC:38230; AA959718; ingls; Tax1 (human T-cell leukemia virus type I) binding protein 2; cDNA sequence BC032215; D8Wsu151e; BC032215; infantile gliosis; TAX1BP2; Tax1bp2; Trx|
|Strain of Origin||DBA/2J|
|General Note||This spontaneous mutation arose in 1991 at The Jackson Laboratory.|
|Molecular Note||Exon 4 contains a thymidine to guanosine transition at position 467 (c.467T>G) that results in an amino acid substitution of arginine for leucine at position 156 (L156R). This allele is hypomorphic.|
|Heterozygous or Wild-type for Vac14<ingls>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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