These mice carry a spontaneous mutation at the Vac14 locus characterized by small size and prevalent hydrocephalus. They are suitable for use in applications related to the study of the phosphoinositide phosphate regulatory complex and neuron development in the mammalian nervous system.
Read More +Genetic Background | Generation |
---|---|
000664 C57BL/6J |
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous (Hypomorph) | Vac14 | Vac14 homolog (S. cerevisiae) |
Mice homozygous for the recessive Vac14ingls mutation are significantly smaller than their littermates, and their coat color is dilute with white underfur both dorsally and ventrally (Sweet 1991; Samples 2003). Most develop hydrocephalus with onset by one week of age. Homozygotes are weak and uncoordinated and have difficulty righting themselves. They fail to gain weight during the second week of life, and most die by three weeks of age. (Bronson et al. 2003) Length of survival appears to correlate with the severity of hydrocephalus, which varies from absent to severe; however, all homozygotes die by four weeks, whether or not they are hydrocephalic (Samples et al. 2003).
Hydrocephalus affects the lateral ventricles most severely; serial sections reveal an intact cerebral aqueduct. Diffuse astrocytic hypertrophy and hyperplasia are observed in brains and spinal cords of mutant mice. A few mutants develop status spongiosis, and a few exhibit gliosis alone. Astrocytes cultured from affected mice stop dividing within three weeks, but survive for up to 12 weeks. They do not develop into tumors when implanted into the brains of normal mice. (Bronson et al. 2003)
ingls arose spontaneously in a breeding colony of the inbred strain DBA/2J at The Jackson Laboratory in 1991. It has been backcrossed onto C57BL/6J and is maintained by crossing hosts of homozygous ovarian transplants to C57BL/6J males, then intercrossing the resultant obligate heterozygotes. In October 2003 this strain had reached generation N19F1.
Allele Name | infantile gliosis |
---|---|
Allele Type | Spontaneous (Hypomorph) |
Allele Synonym(s) | ingls; Vac14L156R |
Gene Symbol and Name | Vac14, Vac14 homolog (S. cerevisiae) |
Gene Synonym(s) | |
Strain of Origin | DBA/2J |
Chromosome | 8 |
General Note | This spontaneous mutation arose in 1991 at The Jackson Laboratory. |
Molecular Note | Exon 4 contains a T-to-C transition at position 467 (c.467T>G) that results in an amino acid substitution of leucine with arginine at position 156 (p.L156R). This allele is hypomorphic. |
When using the infantile gliosis mouse strain in a publication, please cite the originating article(s) and include JAX stock #003095 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Vac14<ingls> |
Frozen Mouse Embryo | B6.D2-Vac14<ingls>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | B6.D2-Vac14<ingls>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | B6.D2-Vac14<ingls>/GrsrJ | $3373.50 |
Frozen Mouse Embryo | B6.D2-Vac14<ingls>/GrsrJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.