These Bcl2 knock-out mice exhibit expression of beta-galactosidase in populations of large sensory neurons. They are suitable for use in applications related to the study of apoptosis.
Michael Meyer, Ludwig-Maximilians-University Munich
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Bcl2 | B cell leukemia/lymphoma 2 |
Heterozygous mice exhibit expression of beta-galactosidase in populations of large sensory neurons. Mice homozygous for the Bcl2tm1Mpin targeted mutation do not produce either the a or b form of the Bcl2 protein. Bcl2 is a major regulator of programmed cell death, a critical process in shaping the developing nervous system. The absence of the Bcl2 does not significantly influence the development of motor neurons before or during the main period of physiological cell death. Rather, Bcl2 exerts its influence beyond this period, subsequent to the phase where the majority of neuronal loss normally takes place. Polycystic kidney disease is less severe in this strain compared to the Bcl2tm1Sjk targeted mutation (Stock No. 002265).
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | large sensory neurons; Bcl2 (B-cell leukemia/lymphoma 2) gene products not produced |
Allele Name | targeted mutation 1, Max-Planck-Institute for Neurobiology |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | bcl-2 - |
Gene Symbol and Name | Bcl2, B cell leukemia/lymphoma 2 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | large sensory neurons; Bcl2 (B-cell leukemia/lymphoma 2) gene products not produced |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | Exon 2 was disrupted by the insertion of a lacZ gene and neomycin selection cassette. Western blot analysis on hippocampal lysates from homozygous mice demonstrated that no stable Bcl2 protein was made from this allele. A beta-galactosidase protein was produced from this allele under the control of the endogenous promoter. |
Mutations Made By | Michael Meyer, Ludwig-Maximilians-University Munich |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to 129S1/SvImJ inbred mice (Stock No. 002448). Homozygous mice are lethal.
When using the bcl-2 - mouse strain in a publication, please cite the originating article(s) and include JAX stock #003082 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Bcl2<tm1Mpin> |
Frozen Mouse Embryo | 129S1/SvImJ-Bcl2<tm1Mpin>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S1/SvImJ-Bcl2<tm1Mpin>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S1/SvImJ-Bcl2<tm1Mpin>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | 129S1/SvImJ-Bcl2<tm1Mpin>/J Frozen Embryo | $3373.50 |
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