129S1/SvImJ-Bcl2^tm1Mpin/J

Stock number: 003082
Product name: bcl-2 -
Research areas: Apoptosis Research, Cancer Research, Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Neurobiology Research, Research Tools

Description

These Bcl2 knock-out mice exhibit expression of beta-galactosidase in populations of large sensory neurons. They are suitable for use in applications related to the study of apoptosis.

Detailed description

Heterozygous mice exhibit expression of beta-galactosidase in populations of large sensory neurons. Mice homozygous for the Bcl2^tm1Mpin targeted mutation do not produce either the a or b form of the Bcl2 protein. Bcl2 is a major regulator of programmed cell death, a critical process in shaping the developing nervous system. The absence of the Bcl2 does not significantly influence the development of motor neurons before or during the main period of physiological cell death. Rather, Bcl2 exerts its influence beyond this period, subsequent to the phase where the majority of neuronal loss normally takes place. Polycystic kidney disease is less severe in this strain compared to the Bcl2^tm1Sjk targeted mutation (Stock No. 002265).

Allele

Symbol: Bcl2^tm1Mpin
Name: targeted mutation 1, Max-Planck-Institute for Neurobiology
MGI accession ID: MGI:1857430
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Bcl2
Marker name: B cell leukemia/lymphoma 2
Chromosome: 1
Strain of origin: 129S2/SvPas
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: large sensory neurons; Bcl2 (B-cell leukemia/lymphoma 2) gene products not produced
Molecular note: Exon 2 was disrupted by the insertion of a lacZ gene and neomycin selection cassette. Western blot analysis on hippocampal lysates from homozygous mice demonstrated that no stable Bcl2 protein was made from this allele. A beta-galactosidase protein was produced from this allele under the control of the endogenous promoter.