These doubly congenic mice carry two chromosomal segments, one bound by an Mit marker and B-cell leukemia/lymphoma 2 (Bcl2) and the other by Mit markers. One segment from Chromosome 17 includes the major histocompatibility complex (H2) and the insulin dependent diabetes susceptibility locus 1 (Idd1), and the other segment from Chromosome 1 contains the insulin dependent diabetes susceptibility locus 5 (Idd5).
Dr. Edward Leiter, The Jackson Laboratory
|Allele Type||Gene Symbol||Gene Name|
|Not Applicable||H2||histocompatibility-2, MHC|
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd1||insulin dependent diabetes susceptibility 1|
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd5||insulin dependent diabetes susceptibility 5|
|Marker Symbol||Marker Name|
|Bcl2||B cell leukemia/lymphoma 2|
|D17Mit10||DNA segment, Chr 17, Massachusetts Institute of Technology 10|
|D17Mit21||DNA segment, Chr 17, Massachusetts Institute of Technology 21|
|D1Mit3||DNA segment, Chr 1, Massachusetts Institute of Technology 3|
This strain is doubly congenic for a 19 cM segment of Chr 17 (called c17 in the reference below) extending from D17Mit21 through D17Mit10 and including the major histocompatibility complex, H2, and the insulin dependent diabetes susceptibility locus Idd1 and for a 44 cM segment of Chr 1 (called c1c by Yui et al.) extending from D1Mit3 through Bcl2 (B-cell leukemia/lymphoma 2) and including Idd5(insulin dependent diabetes susceptibility 5). No information has been published for this double congenic. However, upon histologic examination of the pancreas, a significantly higher percentage of both B6.NOD-D1Mit3-Bcl2 (Idd5) and B6.NOD-D17Mit21-D17Mit10 (H2g7, Idd1) singly congenic mice than of C57BL/6J control mice were found to exhibit periinsulitis, and more extensive mononuclear cell infiltrates were observed in the pancreas of these mice. The pancreatic infiltrates were not associated specifically with the islets. Insulitis (intraislet infiltration) was extremely rare, and no more than one affected islet was observed in a single animal, even in cases where extensive perivascular/periductal infiltrates existed. Although male and female B6.NOD-D17Mit21-D17Mit10 (H2g7, Idd1) mice exhibited similar incidence of pancreatic infiltration, B6.NOD-D1Mit3-Bcl2 (Idd5) females developed periinsulitis at twice the frequency of males. Female NOD mice are more susceptible than males to both insulitis and diabetes.
Genomic segments found in earlier linkage studies to include diabetogenic loci were transferred from NOD/Uf to C57BL/6 by six successive backcrosses (to N7). Sibs of each lineage were then intercrossed to generate homozygotes for each segment. Microsatellite analysis was used to type mice for loci in the regions of interest. As evidence suggested the existence of multiple loci on Chr 1 that affect susceptibility to IDD and/or insulitis, two congenic lines were generated carrying overlapping segments of Chr 1, each including the diabetes susceptibility locus and one insulitis susceptibility locus. The B6.NOD-D1Mit3-Bcl2 D17Mit21-D17Mit10 (Idd5; H2g7 , Idd1) double congenic strain was created by crossing mice of the singly congenic B6.NOD-D17Mit21-D17Mit10 (H2g7 , Idd1) and B6.NOD-D1Mit3-Bcl2 (Idd5) strains, then breeding to homozygosity for both congenic segments.
|Allele Name||g7 variant|
|Allele Type||Not Applicable|
|Gene Symbol and Name||H2, histocompatibility-2, MHC|
|Gene Synonym(s)||H-2; H-2; MHC-II|
|Strain of Origin||Not Applicable|
|General Note||The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2g7|
|Gene Symbol and Name||Idd1, insulin dependent diabetes susceptibility 1|
|Gene Synonym(s)||Idd-1; Idd-1|
|Strain of Origin||NOD/Uf|
|General Note||NOD is homozygous for recessive alleles for susceptibility at all three loci, Idd1s, Idd2s, and Idd3s. The dominant alleles for non-susceptibility to IDD, Idd1r, etc., occur in the NON strain. Homozygosity for the recessive alleles at all three loci is necessary for the development of IDD.The Idd1 locus is linked to the major histocompatibility locus on Chr 17, but Idd2 is on Chr 9 (1) and Idd3 is on Chr 3 (J:8783, J:3351). |
This locus is also linked to peripheral CD4 lymphocytosis.
|Molecular Note||This allele confers increased periinsulitis and increased CD4 lymphocytosis compared to C57BL/6.|
|Gene Symbol and Name||Idd5, insulin dependent diabetes susceptibility 5|
|Gene Synonym(s)||Idd-5; Idd-5|
|Strain of Origin||NOD|
|Molecular Note||This allele confers susceptibility to insulin dependent diabetes compared to C57BL/10.|
|Marker Synonym(s)||AW986256; Bcl-2; Bcl-2; C430015F12Rik; C430015F12Rik; D630044D05Rik; D830018M01Rik; D830018M01Rik; PPP1R50; RIKEN cDNA C430015F12 gene; RIKEN cDNA D630044D05 gene; RIKEN cDNA D830018M01 gene; expressed sequence AW986256|
All animals produced from this C57BL/6 congenic are homozygous for 2 NOD intervals D1Mit3-Bcl2 and D17Mit21 - D17Mit10. When maintaining a live colony, homozygous mice may be bred together.
When using the B6.NOD Idd1,5 (c1c) mouse strain in a publication, please cite the originating article(s) and include JAX stock #003069 in your Materials and Methods section.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.