These doubly congenic mice carry two chromosomal segments, one bound by an Mit marker and B-cell leukemia/lymphoma 2 (Bcl2) and the other by Mit markers. One segment from Chromosome 17 includes the major histocompatibility complex (H2) and the insulin dependent diabetes susceptibility locus 1 (Idd1), and the other segment from Chromosome 1 contains the insulin dependent diabetes susceptibility locus 5 (Idd5).
Dr. Edward Leiter, The Jackson Laboratory
|Allele Type||Gene Symbol||Gene Name|
|Not Applicable||H2||histocompatibility-2, MHC|
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd1||insulin dependent diabetes susceptibility 1|
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd5||insulin dependent diabetes susceptibility 5|
|Marker Symbol||Marker Name|
|Bcl2||B cell leukemia/lymphoma 2|
|D17Mit10||DNA segment, Chr 17, Massachusetts Institute of Technology 10|
|D17Mit21||DNA segment, Chr 17, Massachusetts Institute of Technology 21|
|D1Mit3||DNA segment, Chr 1, Massachusetts Institute of Technology 3|
This strain is doubly congenic for a 19 cM segment of Chr 17 (called c17 in the reference below) extending from D17Mit21 through D17Mit10 and including the major histocompatibility complex, H2, and the insulin dependent diabetes susceptibility locus Idd1 and for a 44 cM segment of Chr 1 (called c1c by Yui et al.) extending from D1Mit3 through Bcl2 (B-cell leukemia/lymphoma 2) and including Idd5(insulin dependent diabetes susceptibility 5). No information has been published for this double congenic. However, upon histologic examination of the pancreas, a significantly higher percentage of both B6.NOD-D1Mit3-Bcl2 (Idd5) and B6.NOD-D17Mit21-D17Mit10 (H2g7, Idd1) singly congenic mice than of C57BL/6J control mice were found to exhibit periinsulitis, and more extensive mononuclear cell infiltrates were observed in the pancreas of these mice. The pancreatic infiltrates were not associated specifically with the islets. Insulitis (intraislet infiltration) was extremely rare, and no more than one affected islet was observed in a single animal, even in cases where extensive perivascular/periductal infiltrates existed. Although male and female B6.NOD-D17Mit21-D17Mit10 (H2g7, Idd1) mice exhibited similar incidence of pancreatic infiltration, B6.NOD-D1Mit3-Bcl2 (Idd5) females developed periinsulitis at twice the frequency of males. Female NOD mice are more susceptible than males to both insulitis and diabetes.
Genomic segments found in earlier linkage studies to include diabetogenic loci were transferred from NOD/Uf to C57BL/6 by six successive backcrosses (to N7). Sibs of each lineage were then intercrossed to generate homozygotes for each segment. Microsatellite analysis was used to type mice for loci in the regions of interest. As evidence suggested the existence of multiple loci on Chr 1 that affect susceptibility to IDD and/or insulitis, two congenic lines were generated carrying overlapping segments of Chr 1, each including the diabetes susceptibility locus and one insulitis susceptibility locus. The B6.NOD-D1Mit3-Bcl2 D17Mit21-D17Mit10 (Idd5; H2g7 , Idd1) double congenic strain was created by crossing mice of the singly congenic B6.NOD-D17Mit21-D17Mit10 (H2g7 , Idd1) and B6.NOD-D1Mit3-Bcl2 (Idd5) strains, then breeding to homozygosity for both congenic segments.
|Allele Name||g7 variant|
|Allele Type||Not Applicable|
|Gene Symbol and Name||H2, histocompatibility-2, MHC|
|Gene Synonym(s)||H-2; H-2; MHC-II|
|Strain of Origin||Not Applicable|
|General Note||The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2g7|
|Gene Symbol and Name||Idd1, insulin dependent diabetes susceptibility 1|
|Gene Synonym(s)||Idd-1; Idd-1|
|Strain of Origin||NOD/Uf|
|General Note||NOD is homozygous for recessive alleles for susceptibility at all three loci, Idd1s, Idd2s, and Idd3s. The dominant alleles for non-susceptibility to IDD, Idd1r, etc., occur in the NON strain. Homozygosity for the recessive alleles at all three loci is necessary for the development of IDD.The Idd1 locus is linked to the major histocompatibility locus on Chr 17, but Idd2 is on Chr 9 (1) and Idd3 is on Chr 3 (J:8783, J:3351). |
This locus is also linked to peripheral CD4 lymphocytosis.
|Molecular Note||This allele confers increased periinsulitis and increased CD4 lymphocytosis compared to C57BL/6.|
|Gene Symbol and Name||Idd5, insulin dependent diabetes susceptibility 5|
|Gene Synonym(s)||Idd-5; Idd-5|
|Strain of Origin||NOD|
|Molecular Note||This allele confers susceptibility to insulin dependent diabetes compared to C57BL/10.|
|Marker Synonym(s)||AW986256; Bcl-2; Bcl-2; C430015F12Rik; C430015F12Rik; D630044D05Rik; D830018M01Rik; D830018M01Rik; PPP1R50; RIKEN cDNA C430015F12 gene; RIKEN cDNA D630044D05 gene; RIKEN cDNA D830018M01 gene; expressed sequence AW986256|
All animals produced from this C57BL/6 congenic are homozygous for 2 NOD intervals D1Mit3-Bcl2 and D17Mit21 - D17Mit10. When maintaining a live colony, homozygous mice may be bred together.
|Please inquire about possible genotypes.|
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