This strain is doubly congenic for a 19 cM segment of Chr 17 (called c17 in the reference below) extending from D17Mit21through D17Mit10and including the major histocompatibility complex, H2,and the insulin dependent diabetes susceptibility locus Idd1and for a 42 cM segment of Chr 11 (called c11 by Yui et al.)extending from Csf2(colony stimulating factor 2 (granulocyte-macrophage)) through D11Mit42and including the insulin dependent diabetes susceptibility locus Idd4.No information has been published for this double congenic strain. However, upon histologic examination of the pancreas, a significantly higher percentage of B6.NOD-(D17Mit21-D17Mit10) singly congenic mice than of C57BL/6J control mice were found to exhibit periinsulitis, and more extensive mononuclear cell infiltrates were observed in the pancreata of these mice. The pancreatic infiltrates were not associated specifically with the islets. Insulitis (intraislet...
|Allele Type||Gene Symbol||Gene Name|
|Not Applicable||H2||histocompatibility-2, MHC|
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd1||insulin dependent diabetes susceptibility 1|
|Allele Type||Gene Symbol||Gene Name|
|QTL||Idd4||insulin dependent diabetes susceptibility 4|
|Marker Symbol||Marker Name|
|Csf2||colony stimulating factor 2 (granulocyte-macrophage)|
|D11Mit42||DNA segment, Chr 11, Massachusetts Institute of Technology 42|
|D17Mit10||DNA segment, Chr 17, Massachusetts Institute of Technology 10|
|D17Mit21||DNA segment, Chr 17, Massachusetts Institute of Technology 21|
This strain is doubly congenic for a 19 cM segment of Chr 17 (called c17 in the reference below) extending from D17Mit21through D17Mit10and including the major histocompatibility complex, H2,and the insulin dependent diabetes susceptibility locus Idd1and for a 42 cM segment of Chr 11 (called c11 by Yui et al.)extending from Csf2(colony stimulating factor 2 (granulocyte-macrophage)) through D11Mit42and including the insulin dependent diabetes susceptibility locus Idd4.No information has been published for this double congenic strain. However, upon histologic examination of the pancreas, a significantly higher percentage of B6.NOD-(D17Mit21-D17Mit10) singly congenic mice than of C57BL/6J control mice were found to exhibit periinsulitis, and more extensive mononuclear cell infiltrates were observed in the pancreata of these mice. The pancreatic infiltrates were not associated specifically with the islets. Insulitis (intraislet infiltration) was extremely rare, and no more than one affected islet was observed in a single animal, even in cases where extensive perivascular/periductal infiltrates existed. Incidence and severity of periinsulitis in singly congenic B6.NOD-(Csf2-D11Mit42) mice were similar to those of controls. Of note, whereas female NOD mice are more susceptible than males to both insulitis and diabetes, male and female mice of the singly congenic B6.NOD-(D17M it21-D17Mit10) strain exhibited similar incidence of pancreatic infiltration.
Genomic segments found in earlier linkage studies to include diabetogenic loci were transferred in the laboratory of E.K. Wakeland from NOD/Uf to C57BL/6 by six successive backcrosses (to N7). Sibs of each lineage were then intercrossed to generate mice homozygous for each segment. Microsatellite analysis was used to type mice for loci in the regions of interest. The B6.NOD-(Csf2-D11Mit42) (D17Mit21-D17Mit10) double congenic strain was created by crossing mice of the singly congenic B6.NOD-(D17Mit21-D17Mit10) and B6.NOD-(Csf2-D11Mit42) strains, then breeding to homozygosity for both congenic segments.
|Allele Name||g7 variant|
|Allele Type||Not Applicable|
|Gene Symbol and Name||H2, histocompatibility-2, MHC|
|Gene Synonym(s)||H-2; H-2; MHC-II|
|Strain of Origin||Not Applicable|
|General Note||The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2g7|
|Gene Symbol and Name||Idd1, insulin dependent diabetes susceptibility 1|
|Gene Synonym(s)||Idd-1; Idd-1|
|Strain of Origin||NOD/Uf|
|General Note||NOD is homozygous for recessive alleles for susceptibility at all three loci, Idd1s, Idd2s, and Idd3s. The dominant alleles for non-susceptibility to IDD, Idd1r, etc., occur in the NON strain. Homozygosity for the recessive alleles at all three loci is necessary for the development of IDD.The Idd1 locus is linked to the major histocompatibility locus on Chr 17, but Idd2 is on Chr 9 (1) and Idd3 is on Chr 3 (J:8783, J:3351). |
This locus is also linked to peripheral CD4 lymphocytosis.
|Molecular Note||This allele confers increased periinsulitis and increased CD4 lymphocytosis compared to C57BL/6.|
|Gene Symbol and Name||Idd4, insulin dependent diabetes susceptibility 4|
|Gene Synonym(s)||Idd-4; Idd-4|
|Strain of Origin||NOD|
|General Note||This allele also confers decreased hepatic iNKT cell numbers on a C57BL/6 background.|
|Molecular Note||This allele confers susceptibility to insulin dependent diabetes compared to C57BL/10.|
|Marker Synonym(s)||Csfgm; Csfgm; GMCSF; Gm-CSf; Gm-csf; Gmcsf; MGI-IGM; colony stimulating factor, granulocyte macrophage|
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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