CBcNO7D/LtJ

Stock number: 003055
Product name: CBNO7D
Research areas: Diabetes and Obesity Research

Description

This strain is one of a series of CBcNO recombinant congenic strains derived from an initial cross between CBA and NOD mice.

Detailed description

Neither spontaneous IDDM nor significant insulitis has been observed in any of the NOcCB or CBcNO recombinant congenic strains. Histologic analysis revealed leukocyte infiltration of the submandibular salivary glands (sialitis) of aging mice. Mice of these strains also developed pancreatic infiltrates, but these were limited to the perivascular and periductal regions of the pancreas (peri-insulitis) and did not focus on the islets. All strains exhibited strong resistance to induction of both IDDM and insulitis by a cyclophosphamide treatment regimen that causes diabetes within two weeks of the second dose in 90% of female NOD mice.

Development

The CBcNO6 recombinant congenic strain was derived from an initial cross between mice of strains CBA/JLsLt and NOD/Shi. The former is "a substrain of CBA/J selected for high incidence of spontaneous...pancreatic necrosis." An F1 female from this mating was backcrossed to a CBA/JLsLt male, and the resultant N2F1 animals were sib-mated. "At F13...CBcNO6 was split into two separate lines, with the new subline designated CBcNO7....At F20, preliminary genome scan analysis of CBcNO7 mice...revealed a small amount of residual heterozygosity on Chrs 2 and 3, permitting selection of four sublines (CBcNO7A, B, C and D) based upon their genotype at D2Mit77 (a marker for the Idd13 region in NOD) and D3Mit100 (a marker for the Idd10/Idd17 region in NOD)....Residual heterozygosity at other loci...was fixed to homozygosity in random fashion." CBcNO7D/Lt is homozygous for the NOD alleles at D2Mit77, on Chr 2, and the Chr 3 loci D3Mit22, Fcgr1 and Amy1.