Overview

Also Known As:Fmr1 KO

These Fmr1 knockout mice may be useful in applications related to the study of Fragile X Syndrome.

Donating Investigator

Ben A Oostra, Erasmus University

IMR Colony, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
001800 FVB/NJ

Fmr1^tm1Cgr

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Fmr1	fragile X mental retardation 1

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Research Applications

Neurobiology Research
Mouse/Human Gene Homologs
Developmental Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

This strain is homozygous for the retinal degeneration allele Pde6b^rd1.

Detailed Description

Mice homozygous for the Fmr1^tm1Cg targeted mutation show macroorchidism (enlarged testes), learning deficits, and hyperactivity. Macroorchidism in caused by an increased rate of Sertoli cell proliferation during embryogenesis which may be independent of FSH signalling. Comparison of homozygotes to wildtype littermates in hidden- and visible-platform water maze learning showed deficits in spatial learning and motor performance. This strain is homozygous for the retinal degeneration allele Pde6b^rd1.

Development

The targeted mutation was made in the laboratory of Dr. Ben Oostra at Erasmus University in the Netherlands by replacing exon 5 of the fragile X mental retardation syndrome 1 (Fmr1) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected to C57BL/6J blastocysts and resulting males were bred to C57BL/6J females. These mice were backcrossed to FVB/N mice for an unknown number of generations. Upon arrival at The Jackson Laboratory, these mice were bred to FVB/NJ (Stock No. 001800) mice for at least 5 generations.

Control Suggestions

001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Bakker CE; Verheij C; Willemsen R; Vanderhelm R; Oerlemans F; Vermey M; Bygrave A; Hoogeveen AT; Oostra BA; Reyniers E; Deboulle K; Dhooge R; Cras P; Vanvelzen D; Nagels G; Martin JJ; Dedyn PP; Darby JK; Willems PJ; The Dutch-Belgium Fragile X Consortium. 1994. Fmr1 knockout mice: a model to study fragile x mental retardation. The Dutch-Belgium Fragile X Consortium Cell 78(1):23-33PubMed: 8033209 MGI: J:19220

View All References

Genetics

Fmr1^tm1Cgr

Allele Symbol: Fmr1^tm1Cgr

Allele Name	targeted mutation 1, Ben Oostra
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Fmr1 KO; Fmr1^tm4Cgr; FMRP KO; fmr-tm1Cgr; FraX
Gene Symbol and Name	Fmr1, fragile X mental retardation 1
Gene Synonym(s)
Strain of Origin	129P2/OlaHsd
Chromosome	X
General Note	Genbank: AF179463 and AF170530
Molecular Note	A neomycin resistance gene was inserted into exon 5. RT-PCR analysis on testis RNA derived from hemizygous male mice demonstrated that no detectable transcript was produced from this allele, and western blot analysis on extracts of testes, liver, kidney and brain of hemizygous male mice confirmed that no stable encoded protein was made.
Mutations Made By	Ben Oostra, Erasmus University

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

fragile X syndrome

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

autism spectrum disorder

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Fmr1^tm1Cgr related

Neurobiology Research
- Fragile X Mental Retardation Syndrome
- Behavioral and Learning Defects
Mouse/Human Gene Homologs
- fragile site mental retardation 1
Developmental Biology Research
- Internal/Organ Defects
  - multiple

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Fmr1^tm1Cgr/Y
B6.129P2-Fmr1

behavior/neurological phenotype

hyperactivity
- during the light cycle

hypoactivity
- during the dark cycle

hypodipsia
- during the dark cycle, mice exhibit less daily water ingestion with decreased water ingested per lick compared with wild-type mice

abnormal circadian behavior
- mice exhibit less activity during the dark cycle but more bouts of activity during the light cycle compared with wild-type mice

endocrine/exocrine gland phenotype

increased testis weight
- testes are significantly heavier than wild-type

reproductive system phenotype

increased testis weight
- testes are significantly heavier than wild-type

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * FVB

growth/size/body region phenotype

increased body weight
- body weight was significantly increased in one of the two cohorts tested

mammalian phenotype

vision/eye phenotype
- Normal - mice are wild-type at the Pde6b locus and are therefore not blind

nervous system phenotype

abnormal hippocampal mossy fiber morphology
- density of Timm granules (Timm staining identifies neural elements that contain heavy metals) in zinc-rich mossy fiber terminals is increased in inner molecular layer and close to the hilus in the stratum oriens

convulsive seizures
- some mice exhibit bilateral motor seizures that range from head twitching to forelimb and hindlimb clonus, loss of postural control and tonic twisting of head and torso

tonic-clonic seizures
- observed in some mice

behavior/neurological phenotype

decreased anxiety-related response
- total number of entries in arms of elevated plus maze was significantly decreased as compared to littermate control in one of the two cohorts tested

social withdrawal
- mice spend more time in an empty cage than in a cage with a strange mouse in sociability choice test

increased exploration in new environment
- distance traveled in open field test is increased as compared to control

impaired coordination
- Background Sensitivity - mice on the C57BL/6 background have longer latencies on the rotarod test than mice on the mixed FVB and 129P2 background, however, the difference between mutant and control on the mixed FVB and 129P2 background is not significant

increased anxiety-related response
- Background Sensitivity - background is not significant
- distance traveled in open field test is increased as compared to control
- Background Sensitivity - percentage of entries and percentage of time spent in arms of elevated plus maze is significantly increased as compared to Fmr1^tm1Cgr mice on the C57BL/6 background, however, the difference between mutant and control on the mixed FVB and 129P2 background is not significant

tonic-clonic seizures
- observed in some mice

convulsive seizures
- some mice exhibit bilateral motor seizures that range from head twitching to forelimb and hindlimb clonus, loss of postural control and tonic twisting of head and torso

Genotype: Fmr1^tm1Cgr/Y
FVB.129P2-Pde6b<+> Tyr Fmr1/J

nervous system phenotype

increased prepulse inhibition
- Background Sensitivity - magnitude of prepulse inhibition is enhanced although the effect is more prominent on the C57BL/6 background as compared to the FVB background

behavior/neurological phenotype

abnormal locomotor activation
- overall locomotor activity is increased as compared to wild-type
- Background Sensitivity - mice on the FVB background are more active as compared to mice on the C57BL/6 background

abnormal response to novelty
- Background Sensitivity - mice on the C57BL/6 background, but not the FVB background, exhibit an increased tendency to attack a juvenile stimulus mouse
- mice do not explore the novelty stimulus mouse in the social novelty preference test

abnormal social investigation
- Background Sensitivity - mice spend less time in non-social activities; the effect is more pronounced on the FVB background as compared to C57BL/6
- mice do not explore the novelty stimulus mouse in the social novelty preference test
- Background Sensitivity - mice spend less time in affiliative behaviors; the effect is more pronounced on the C57BL/6 background as compared to FVB

decreased startle reflex
- Background Sensitivity - mice on the C57BL/6 background exhibit stronger responses to 100 dBa pulse and lower responses to the 120 dBa pulse as compared to FVB
- startle response is less prominent with increased pulse intensity as compared to wild-type

increased exploration in new environment
- Background Sensitivity - levels of spontaneous alternation and entries into maze arms are increased on the FVB background as compared to the C57BL/6 background
- Normal - however, exploration does not differ between mutant and wild-type

abnormal vocalization
- Background Sensitivity - mice exhibit an increase in the number of vocalization and duration on FVB background as compared to the C57BL/6 background

Genotype: Fmr1^tm1Cgr/Fmr1^tm1Cgr
B6.129P2-Fmr1

behavior/neurological phenotype

impaired conditioned place preference behavior
- for a scent-paired chamber

increased vertical activity

hyperactivity

abnormal object recognition memory
- mice exhibit reduced novel object recognition compared with wild-type mice

decreased aggression towards mice
- mice exhibit less social dominance compared with wild-type mice

growth/size/body region phenotype

increased body weight

mammalian phenotype

behavior/neurological phenotype
- Background Sensitivity - mice on the C57BL/6 background, but not the FVB/NJ background, location of hidden escape platform in water maze after 6 days of training

Genotype: Fmr1^tm1Cgr/Y
B6.129P2-Fmr1/Nwu

mammalian phenotype

behavior/neurological phenotype
- Background Sensitivity - mice exhibit an increase in the number of vocalization and duration on FVB background as compared to the C57BL/6 background
- Background Sensitivity - levels of spontaneous alternation and entries into maze arms are increased on the FVB background as compared to the C57BL/6 background, however, exploration does not differ between mutant and wild-type

nervous system phenotype

increased prepulse inhibition
- Background Sensitivity - magnitude of prepulse inhibition is enhanced although the effect is more prominent on the C57BL/6 background as compared to the FVB background

behavior/neurological phenotype

abnormal social investigation
- Background Sensitivity - mice spend less time in affiliative behaviors; the effect is more pronounced on the C57BL/6 background as compared to FVB
- mice do not preferentially explore the novelty stimulus mouse as compared to the familiar mouse in the social novelty preference test
- Background Sensitivity - mice spend less time in non-social activities; the effect is more pronounced on the FVB background as compared to C57BL/6

decreased startle reflex
- startle response is less prominent with increased pulse intensity as compared to wild-type
- Background Sensitivity - mice on the C57BL/6 background exhibit stronger responses to 100 dBa pulse and lower responses to the 120 dBa pulse as compared to FVB

increased aggression towards mice
- Background Sensitivity - mice on the C57BL/6 background, but not the FVB background, exhibit an increased tendency to attack a juvenile stimulus mouse

abnormal locomotor activation
- Background Sensitivity - mice on the FVB background are more active as compared to the C57BL/6 background
- overall locomotor activity is increased as compared to wild-type

abnormal response to novelty
- mice do not preferentially explore the novelty stimulus mouse as compared to the familiar mouse in the social novelty preference test

increased grooming behavior
- Background Sensitivity - mice spend more time self-grooming; the effect is more pronounced on the C57BL/6 background as compared to the FVB background

Genotype: Fmr1^tm1Cgr/Fmr1^tm1Cgr
involves: 129P2/OlaHsd

behavior/neurological phenotype

increased startle reflex
- in all eye blink conditioning training session the percentage and peak amplitudes of the startle responses were higher

nervous system phenotype

abnormal dentate gyrus morphology
- increased volume of the dentate gyrus (DG) in mutant mice

abnormal long term depression
- induction of long term depression in Purkinje cells is significantly enhanced when stimulating parallel fibers

abnormal neuron differentiation
- exogenously expressed wild-type gene, but not mutant (I304N) rescues both the neuronal and the astrocyte differentiation deficits in homozygous mutant cells
- 60.4% decrease in neuronal differentiation compared with wild-type isolated adult neural progenitor/stem cells (aNPCs)
- 74.9% increase in astrocyte differentiation compared with wild-type isolated adult neural progenitor/stem cells (aNPCs)
- reduced (10.4% ) neuronal differentiation but greater (75.7%) glial differentiation in aNPCs residing in the DG compared with wild-type mice

abnormal neuronal precursor proliferation
- Normal - normal proliferation of astrocytes in the DG of mutant mice
- 11% more cells in mitotic (G2/M) phase compared with wild-type controls
- increased proliferation of isolated aNPCs from both the forebrain and DG of adult homozygous mice
- increased proliferation of both stem and progenitor cells in the DG and subventricular zone of mutant mice

abnormal Purkinje cell morphology
- the percentage of single climbing fiber innervation is increased, the length of spine heads and necks is increased, and spines are more irregular

cellular phenotype

abnormal neuronal precursor proliferation
- increased proliferation of isolated aNPCs from both the forebrain and DG of adult homozygous mice
- 11% more cells in mitotic (G2/M) phase compared with wild-type controls
- increased proliferation of both stem and progenitor cells in the DG and subventricular zone of mutant mice
- Normal - normal proliferation of astrocytes in the DG of mutant mice

abnormal neuron differentiation
- 74.9% increase in astrocyte differentiation compared with wild-type isolated adult neural progenitor/stem cells (aNPCs)
- exogenously expressed wild-type gene, but not mutant (I304N) rescues both the neuronal and the astrocyte differentiation deficits in homozygous mutant cells
- reduced (10.4% ) neuronal differentiation but greater (75.7%) glial differentiation in aNPCs residing in the DG compared with wild-type mice
- 60.4% decrease in neuronal differentiation compared with wild-type isolated adult neural progenitor/stem cells (aNPCs)

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * C57BL/6 * FVB/N

nervous system phenotype

abnormal nerve fiber response
- brief monocular deprivation results in substantial open-eye potentiation rather than the expected deprived-eye depression

abnormal dendritic spine morphology
- dendritic spine length is increased
- density of dendritic spines along apical dendrites of layer V pyramidal cells is increased
- elongated spines are more prevalent in mutant mice
- mice have fewer short mushroom-shaped spines than wild-type

abnormal dendrite morphology
- dendritic spine density is increased in comparison to wildtype

audiogenic seizures
- 72% of mice have a seizure in response to the test tone

reproductive system phenotype

increased testis weight
- increase in weight is only observed in adult (11-12 weeks)

behavior/neurological phenotype

impaired passive avoidance behavior
- mice exhibit reduced latency to enter box 24 hours after initiation of inhibitory avoidance test as compared to wildtype (inhibitory avoidance extinction behavior)

audiogenic seizures
- 72% of mice have a seizure in response to the test tone

endocrine/exocrine gland phenotype

increased testis weight
- increase in weight is only observed in adult (11-12 weeks)

growth/size/body region phenotype

increased body weight
- 10% increase in body weight is observed by postnatal day 26, but is similar to wildtype by day 45

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * C57BL/6J

behavior/neurological phenotype

abnormal anxiety-related response
- increased transition between light and dark areas in a light-dark box assay

hyperactivity
- mutants show significantly more crossings through three infrared beams in an empty cage over 40 min

increased exploration in new environment
- mutants exhibit more exploratory behavior than controls, displaying more line crossings in the lit compartment

increased thermal nociceptive threshold

abnormal spatial learning
- mutants do not seem to be impaired in the retrieval of spatial and nonspatial information in training and reversal trials once this information has been learned, but they are impaired in their acquisition of the reversal task

decreased startle reflex

integument phenotype

increased thermal nociceptive threshold

nervous system phenotype

abnormal retinal rod cell outer segment morphology
- density and linear organization of disks in the rod outer segment are altered

increased prepulse inhibition

abnormal dendritic spine morphology
- hippocampal and cortical neurons exhibit increased density of think (immature) spines compared with wild-type neurons

reproductive system phenotype

enlarged testis
- macroorchidism develops over time

increased testis weight
- weight of testis increases over time, however no structural abnormalities are observed

vision/eye phenotype

abnormal retina morphology
- the number of immature retinal neurons presenting exuberant and disorganized dendrites is increased
- pre- and post-synaptic proteins are deregulated in the retina indicating synaptic alternations
- rhodopsin content is decreased in the retina
- Normal - however, retinal layer thickness is normal

decreased a-wave amplitude
- maximal a wave amplitude is decreased by 26%
- Normal - however, a wave latency is not affected

abnormal retinal rod cell outer segment morphology
- density and linear organization of disks in the rod outer segment are altered

decreased b-wave amplitude
- maximal b wave amplitude is lower and there is an increase in the slope of the curve in its linear part

endocrine/exocrine gland phenotype

enlarged testis
- macroorchidism develops over time

increased testis weight
- weight of testis increases over time, however no structural abnormalities are observed

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd

behavior/neurological phenotype

abnormal eye blink conditioning behavior
- the percentage of conditioned responses was reduced in the 2nd - 4th training sessions and peak amplitude and peak velocity were reduced in the 3rd and 4th training sessions

abnormal spatial learning
- in the reversal trials of the Morris water maze, mutants take more time to escape to the platform than controls

increased startle reflex
- in all eye blink conditioning training session the percentage and peak amplitudes of the startle responses were higher

reproductive system phenotype

increased testis weight

enlarged testis
- macroorchidism decreases somewhat after 168-170 days of age
- progressive enlargement resulting in testes that were 34% bigger than those of wild-type by 168-170 days of age

endocrine/exocrine gland phenotype

enlarged testis
- macroorchidism decreases somewhat after 168-170 days of age
- progressive enlargement resulting in testes that were 34% bigger than those of wild-type by 168-170 days of age

increased testis weight

nervous system phenotype

abnormal Purkinje cell morphology
- the percentage of single climbing fiber innervation is increased, the length of spine heads and necks is increased, and spines are more irregular

abnormal long term depression
- induction of long term depression in Purkinje cells is significantly enhanced when stimulating parallel fibers

Genotype: Fmr1^tm1Cgr/Fmr1^tm1Cgr
involves: 129P2/OlaHsd * C57BL/6J

behavior/neurological phenotype

increased exploration in new environment
- mutants exhibit more exploratory behavior than controls, displaying more line crossings in the lit compartment

hyperactivity
- mutants show significantly more crossings through three infrared beams in an empty cage over 40 min

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * FVB/NJ

behavior/neurological phenotype

abnormal spatial learning
- Background Sensitivity - in contrast, mice on the C57BL/6 background learned platform location and performed similar to wild-type
- mice do not learn location of hidden escape platform in water maze after 6 days of training

endocrine/exocrine gland phenotype

increased testis weight
- testes are significantly heavier than wild-type

mammalian phenotype

vision/eye phenotype
- Normal - mice have at least one wild-type allele of Pde6b and therefore lack vison impairments commonly seen in FVB mice

reproductive system phenotype

increased testis weight
- testes are significantly heavier than wild-type

The following phenotype relates to a compound genotype created using this strain

Genotype: Fmr1^tm1Cgr/Y
either: FVB.129P2(B6)-Fmr1/J or FVB;129P2(B6)-Fmr1/J

behavior/neurological phenotype

audiogenic seizures
- loud (115 dB) sound results in seizures
- seizures are age dependent and are not fully penetrant
- no seizures occur before 10 weeks of age
- seizures begin within 20-30 seconds and are characterized by wild running, erratic leaping, clonic convulsions and progress to tonic hindlimb extension, respiratory arrest and death
- 57% of mice exhibit seizures between 10-12 weeks; 70% of mice exhibit seizures between 20-34 weeks of age

decreased startle reflex

nervous system phenotype

audiogenic seizures
- no seizures occur before 10 weeks of age
- seizures are age dependent and are not fully penetrant
- seizures begin within 20-30 seconds and are characterized by wild running, erratic leaping, clonic convulsions and progress to tonic hindlimb extension, respiratory arrest and death
- 57% of mice exhibit seizures between 10-12 weeks; 70% of mice exhibit seizures between 20-34 weeks of age
- loud (115 dB) sound results in seizures

increased prepulse inhibition
- both a low (75 dB) and higher (85 dB) prepulse inhibits startle response more efficiently as compared to wild-type

Genotype: Fmr1^tm1Cgr/Y
FVB.129P2(B6)-Fmr1/J

behavior/neurological phenotype

decreased startle reflex
- startle response development is normal (at 118 dB) until 3-4 weeks of age, but does not increase as compared to controls

Genotype: Fmr1^tm1Cgr/Y
FVB.129P2(B6)-Pde6b<+> Fmr1

behavior/neurological phenotype

impaired cued conditioning behavior
- mice exhibit reduced average freezing within the intertrial intervals
- mice exhibit reduced freezing compared to controls following CS-US pairings in trace fear conditioning paradigm
- Normal - there are no significant differences in locomotor activity, nociceptive responses and anxiety-like behaviors between mutants and controls

abnormal associative learning

mammalian phenotype

vision/eye phenotype
- Normal - mice are wild-type for Pde6b and therefore sighted

nervous system phenotype

reduced long term potentiation
- synaptic potentiation is blocked in anterior cingulated cortex (ACC) and lateral amygdala (LA) as determined by whole cell patch-clamp recordings,
- Normal - however, short-term synaptic plasticity and basal synaptic transmission are normal

References

Bakker CE; Verheij C; Willemsen R; Vanderhelm R; Oerlemans F; Vermey M; Bygrave A; Hoogeveen AT; Oostra BA; Reyniers E; Deboulle K; Dhooge R; Cras P; Vanvelzen D; Nagels G; Martin JJ; Dedyn PP; Darby JK; Willems PJ; The Dutch-Belgium Fragile X Consortium. 1994. Fmr1 knockout mice: a model to study fragile x mental retardation. The Dutch-Belgium Fragile X Consortium Cell 78(1):23-33PubMed: 8033209 MGI: J:19220

Additional References

Chen L; Toth M. 2001. Fragile X mice develop sensory hyperreactivity to auditory stimuli. Neuroscience 103(4):1043-50PubMed: 11301211 MGI: J:85912
Comery TA; Harris JB; Willems PJ; Oostra BA; Irwin SA; Weiler IJ; Greenough WT. 1997. Abnormal dendritic spines in fragile X knockout mice: maturation and pruning deficits. Proc Natl Acad Sci U S A 94(10):5401-4PubMed: 9144249 MGI: J:70399
Godfraind JM; Reyniers E; De Boulle K; D'Hooge R; De Deyn PP; Bakker CE; Oostra BA; Kooy RF; Willems PJ. 1996. Long-term potentiation in the hippocampus of fragile X knockout mice. Am J Med Genet 64(2):246-51PubMed: 8844057 MGI: J:34552
Kooy RF; D'Hooge R; Reyniers E; Bakker CE; Nagels G; De Boulle K; Storm K; Clincke G; De Deyn PP; Oostra BA; Willems PJ. 1996. Transgenic mouse model for the fragile X syndrome. Am J Med Genet 64(2):241-5PubMed: 8844056 MGI: J:34449
Oostra BA; Willems PJ. 1995. A fragile gene. Bioessays 17(11):941-7PubMed: 8526888 MGI: J:41538
Paradee W; Melikian HE; Rasmussen DL; Kenneson A; Conn PJ; Warren ST. 1999. Fragile X mouse: strain effects of knockout phenotype and evidence suggesting deficient amygdala function. Neuroscience 94(1):185-92PubMed: 10613508 MGI: J:59781
Qin M; Kang J; Smith CB. 2002. Increased rates of cerebral glucose metabolism in a mouse model of fragile X mental retardation. Proc Natl Acad Sci U S A 99(24):15758-63PubMed: 12427968 MGI: J:80518
Reyniers E; Van Bockstaele DR; De Boulle K; Kooy RF; Bakker CE; Oostra BA; Willems PJ. 1996. Mean corpuscular hemoglobin is not increased in Fmr1 knockout mice. Hum Genet 97(1):49-50PubMed: 8557260 MGI: J:31093
Slegtenhorst-Eegdeman KE; de Rooij DG; Verhoef-Post M; van de Kant HJ; Bakker CE; Oostra BA; Grootegoed JA; Themmen AP. 1998. Macroorchidism in FMR1 knockout mice is caused by increased Sertoli cell proliferation during testicular development. Endocrinology 139(1):156-62PubMed: 9421410 MGI: J:44826

Additional - Fmr1^tm1Cgr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Fmr1
- Standard PCR:Fmr1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous females can be bred with hemizygous males. Fmr1 is an X linked gene. The expected coat color from breeding is albino.
- Additional Breeding and Husbandry Support
Appearance
- albino
  Related Genotype: Tyr^c/Tyr^c
Citation
When using the Fmr1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003024 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Fmr1<tm1Cgr>

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Fmr1 KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Fmr1tm1Cgr

Allele Symbol: Fmr1tm1Cgr

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Fmr1tm1Cgr related

Mammalian Phenotype Terms by Genotype

Genotype: Fmr1tm1Cgr/YB6.129P2-Fmr1

behavior/neurological phenotype

endocrine/exocrine gland phenotype

reproductive system phenotype

Genotype: Fmr1tm1Cgr/Yinvolves: 129P2/OlaHsd * FVB

growth/size/body region phenotype

mammalian phenotype

nervous system phenotype

behavior/neurological phenotype

Genotype: Fmr1tm1Cgr/YFVB.129P2-Pde6b<+> Tyr Fmr1/J

nervous system phenotype

behavior/neurological phenotype

Genotype: Fmr1tm1Cgr/Fmr1tm1CgrB6.129P2-Fmr1

behavior/neurological phenotype

growth/size/body region phenotype

mammalian phenotype

Genotype: Fmr1tm1Cgr/YB6.129P2-Fmr1/Nwu

mammalian phenotype

nervous system phenotype

behavior/neurological phenotype

Genotype: Fmr1tm1Cgr/Fmr1tm1Cgrinvolves: 129P2/OlaHsd

behavior/neurological phenotype

nervous system phenotype

cellular phenotype

Genotype: Fmr1tm1Cgr/Yinvolves: 129P2/OlaHsd * C57BL/6 * FVB/N

nervous system phenotype

reproductive system phenotype

behavior/neurological phenotype

endocrine/exocrine gland phenotype

growth/size/body region phenotype

Genotype: Fmr1tm1Cgr/Yinvolves: 129P2/OlaHsd * C57BL/6J

behavior/neurological phenotype

integument phenotype

nervous system phenotype

reproductive system phenotype

vision/eye phenotype

endocrine/exocrine gland phenotype

Genotype: Fmr1tm1Cgr/Yinvolves: 129P2/OlaHsd

behavior/neurological phenotype

reproductive system phenotype

endocrine/exocrine gland phenotype

nervous system phenotype

Genotype: Fmr1tm1Cgr/Fmr1tm1Cgrinvolves: 129P2/OlaHsd * C57BL/6J

behavior/neurological phenotype

Genotype: Fmr1tm1Cgr/Yinvolves: 129P2/OlaHsd * FVB/NJ

behavior/neurological phenotype

endocrine/exocrine gland phenotype

mammalian phenotype

reproductive system phenotype

Genotype: Fmr1tm1Cgr/Yeither: FVB.129P2(B6)-Fmr1/J or FVB;129P2(B6)-Fmr1/J

behavior/neurological phenotype

nervous system phenotype

Genotype: Fmr1tm1Cgr/YFVB.129P2(B6)-Fmr1/J

behavior/neurological phenotype

Genotype: Fmr1tm1Cgr/YFVB.129P2(B6)-Pde6b<+> Fmr1

behavior/neurological phenotype

mammalian phenotype

nervous system phenotype

References

Additional References

Fmr1^tm1Cgr

Allele Symbol: Fmr1^tm1Cgr

Genotype: Fmr1^tm1Cgr related

Genotype: Fmr1^tm1Cgr/Y
B6.129P2-Fmr1

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * FVB

Genotype: Fmr1^tm1Cgr/Y
FVB.129P2-Pde6b<+> Tyr Fmr1/J

Genotype: Fmr1^tm1Cgr/Fmr1^tm1Cgr
B6.129P2-Fmr1

Genotype: Fmr1^tm1Cgr/Y
B6.129P2-Fmr1/Nwu

Genotype: Fmr1^tm1Cgr/Fmr1^tm1Cgr
involves: 129P2/OlaHsd

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * C57BL/6 * FVB/N

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * C57BL/6J

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd

Genotype: Fmr1^tm1Cgr/Fmr1^tm1Cgr
involves: 129P2/OlaHsd * C57BL/6J

Genotype: Fmr1^tm1Cgr/Y
involves: 129P2/OlaHsd * FVB/NJ

Genotype: Fmr1^tm1Cgr/Y
either: FVB.129P2(B6)-Fmr1/J or FVB;129P2(B6)-Fmr1/J

Genotype: Fmr1^tm1Cgr/Y
FVB.129P2(B6)-Fmr1/J

Genotype: Fmr1^tm1Cgr/Y
FVB.129P2(B6)-Pde6b<+> Fmr1

Additional - Fmr1^tm1Cgr related