A C57BL/6J congenic version of this strain is available as Stock No. 007004.
When these Camk2a-tTA transgenic mice are mated to strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene can be blocked by administration of the tetracycline analog, doxycycline. These mice are a "Tet-Off" tool that allow the inducible expression of genes in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's, Parkinson's disease, or other neurodegenerative diseases.
As described for Stock No. 007004, the CaMK2a-tTA transgene integrated into chromosome 12 causing a 508 Kb deletion that spans the 3' half of Vipr2, the entire Wdr60, Esyt2, D430020J02Rik and Ncapg2 loci and the first two exons of Ptprn2. Homozygous mice will therefore have a functional knock-out of the deleted loci, and altered or null expression of Vipr2 and Ptprn2. Founder line 1 has >20 transgene copies [Goodwin et al. 2019 Genome Res. 29:494].
Dr. Mark Mayford, The Scripps Research Institute
Genetic Background | Generation |
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|
Allele Type |
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Transgenic (Null/Knockout, Transactivator) |
CaMK2a-tTA transgenic mice express the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter.
As described for Stock No. 007004, the CaMK2a-tTA transgene integrated into chromosome 12 causing a 508.12 Kb deletion that spans the 3' half of Vipr2 (vasoactive intestinal peptide receptor 2), the entire Wdr60 (WD repeat domain 60), Esyt2 (extended synaptotagmin-like protein 2), D430020J02Rik (RIKEN cDNA D430020J02 gene) and Ncapg2 (non-SMC condensin II complex, subunit G2) loci and the first two exons of Ptprn2 (protein tyrosine phosphatase, receptor type, N polypeptide 2). Homozygous mice will therefore have a functional knock-out of the deleted loci (Wdr60, Esyt2, D430020J02Rik, Ncapg2), and altered or null expression of Vipr2 and Ptprn2. Founder line 1 has a copy number of greater than 20 [Goodwin et al. 2019 Genome Res. 29:494].
Hemizygous mice are viable and fertile. When hemizygotes are mated to a second strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene can be blocked by administration of the tetracycline analog, doxycycline (dox). These mice are a "Tet-Off" tool that allow the inducible expression of genes in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's disease (when used in conjunction with Stock No. 005706, Stock No. 007049, Stock No. 007051, Stock No. 007052), Parkinson's disease (Stock No. 013583), or other neurodegenerative diseases.
Of note, mice expressing Tg(Camk2a-tTA)1Mmay on the C57BL/6 background exhibit resistance to tTA-induced neurotoxicity (dentate gyrus granule cell layer atrophy). All other backgrounds tested (FVB/NJ, CBA/J, 129X1/SvJ, C3H/HeJ, DBA/1J) exhibit varying levels of neurotoxicity (Han et al. 2012 J Neurosci 32:10574).
A transgenic construct was designed with 8.5 kb of the mouse CaMKIIalpha promoter placed upstream of the tetracycline-regulated transactivator (tTA or Tet-Off) gene (flanked by an artificial intron and splice sites at the 5' end and by a polyadenylation signal from SV40 at the 3' end). Founder line B was established and maintained on a mixed B6;CBA genetic background upon its arrival at The Jackson Laboratory.
As described for Stock No. 007004, the CaMK2a-tTA transgene integrated into chromosome 12 causing a 508.12 Kb deletion that spans the 3' half of Vipr2 (vasoactive intestinal peptide receptor 2), the entire Wdr60 (WD repeat domain 60), Esyt2 (extended synaptotagmin-like protein 2), D430020J02Rik (RIKEN cDNA D430020J02 gene) and Ncapg2 (non-SMC condensin II complex, subunit G2) loci and the first two exons of Ptprn2 (protein tyrosine phosphatase, receptor type, N polypeptide 2). Homozygous mice will therefore have a functional knock-out of the deleted loci (Wdr60, Esyt2, D430020J02Rik, Ncapg2), and altered or null expression of Vipr2 and Ptprn2. Founder line 1 has a copy number of greater than 20 [Goodwin et al. 2019 Genome Res. 29:494].
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
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Site of Expression | Expresses tTA in forebrain neurons. |
Allele Name | transgene insertion 1, Mark Mayford |
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Allele Type | Transgenic (Null/Knockout, Transactivator) |
Allele Synonym(s) | CamDAT; CaMKIIalpha-tTA; CaMKII-tTA; CAMK-rTA; line B; pCaMKII-tTA; Tg(CaMKIItTA)Mmay; Tgalpha-CaMkII-tTA |
Gene Symbol and Name | Tg(Camk2a-tTA)1Mmay, transgene insertion 1, Mark Mayford |
Gene Synonym(s) | |
Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | Expresses tTA in forebrain neurons. |
Strain of Origin | Not Specified |
Chromosome | 12 |
General Note | This transgene is line B. Transgenic mice are viable, fertile, and display no overt phenotypic defects. Administration of tetracycline analogs such as doxycycline blocks transgene expression. |
Molecular Note | The transgene contains the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain specific calcium/calmodulin-dependent kinase II promoter. The transgene integrated into chromosome 12 causing a 508.12 Kb deletion and disrupting the following genes: Vipr2, Wdr60, Esyt2, Ncapg2, and Ptprn2. The deletion results in a functional knock-out of Vipr2, Wdr60, Esyt2, and Ncapg2 in homozygous mice. Founder line 1 has a copy number of greater than 20. |
Mutations Made By | Dr. Mark Mayford, The Scripps Research Institute |
Expected coat color from breeding is Black, Agouti. When maintained in a live colony, these mice were bred as wildtype sib x hemizygous or reciprocal.
When using the CaMKII-tTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #003010 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Camk2a-tTA)1Mmay |
Frozen Mouse Embryo | B6;CBA-Tg(Camk2a-tTA)1Mmay/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Camk2a-tTA)1Mmay/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Camk2a-tTA)1Mmay/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;CBA-Tg(Camk2a-tTA)1Mmay/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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