BPH/2J

Stock number: 003005
Product name: Blood Pressure High
Research areas: Cardiovascular Research

Description

The hypertensive BPH/2 mice have elevated systolic blood pressures at five weeks, compared to the co-selected normotensive BPN/3 (#003004) and low blood pressure BPL/1 (#003006) inbred strains.

Detailed description

The hypertensive BPH/2 mice have elevated systolic blood pressures at five weeks and by 150 days differ from the BPL/1strain (#003006) by 60 mmHg. The BPH/2 strain has a higher heart rate, larger hearts and kidneys, and higher hematocrits than the BPL/1. It also has lower renin, aldosterone and angiotensin I levels compared to the BPL/1 and BPN/3 (#003004). The original HBP (high blood pressure) and LBP (low blood pressure) selected lines showed a number of biochemical and physiological differences which have not been reexamined in the inbred strains. These include differences in brain catecholamines, calmodulin concentrations, heat sensitivity, alcohol preference, and longevity. The differences in longevity were striking. The hypotensive selected lines lived two to three hundred days longer, on average, than the hypertensive selected line. Biometrical genetic analysis suggested that three to five genes are responsible for the difference in blood pressure between the BPH/2 and BPL/1. A recent genome scan of an F2 from a BPH/2 x BPL/1 cross found three chromosome locations that cosegregated with blood pressure. Two of these sites were verified by candidate gene cosegregation: angiotensinogen on chromosome 8 and mouse kallikrein binding protein on chromosome 12.

Development

In 1964, a two-way selection program was initiated at the Jackson Laboratory to produce hypertensive and hypotensive mice. These colonies were moved to the University of Kansas in 1970, where the selection program continued for a total of 30 generations. The maximum divergence between the two selection lines, HBP and LBP, was 60 mmHg. Brother-sister matings followed the selection program to produce the inbred hypertensive BPH/2 and hypotensive BPL/1 strains. A non-selected control was maintained throughout the selection program; brother-sister matings from this line produced the BPN/3 normotensive strain. These three strains were returned to The Jackson Laboratory in 1997.