Homozygous Baxtm1Sjk mice display aberrant apoptosis with hyperplasia displayed in various tissues, including thymocytes, B cells, and cells of the reproductive organs.
Dr. Stanley J. Korsmeyer, Dana-Farber Cancer Institute
Mice homozygous for the Baxtm1Sjk mutation are viable but display lineage-specific aberrations in cell death. Thymocytes and B cells from homozygous mutant mice display hyperplasia. Ovaries contain unusual atretic follicles with excess granulosa cells while Bax-deficient males are infertile. There is an accumulation of atypical premeiotic germ cells and no mature haploid sperm found in seminiferous tubules. Multinucleated giant cells and dysplastic cells accompany massive cell death.
Used in conjunction with strain B6.129-Bak1tm1Thsn/J (see Stock No. 004183), to generate the double knock-out Bak/Bax, a model for demonstrating severe defects in the regulation of apoptosis during development and tissue homeostasis.
Coat color of Baxtm1Sjk mice
The coat color loci tyrosinase (Tyr) and pink-eyed dilution (p) are linked to the Bcl2-associated X protein (Bax) gene. According to backcross data from the Mouse Genome Database, Bax is at 23.00 cM, p is at 28.00 cM, and Tyr is at 44.00 cM on chromosome 7. The targeted disruption of the Bax gene was performed in a 129-derived RW-4 ES cell line (Aw/Aw p Tyrc-ch/p Tyrc) and has been backcrossed 8 generations to C57BL/6 (a/a +p +Tyr-c/+p +Tyr-c; nonagouti black). Statistically, C57BL/6J-Baxtm1Sjk mice (Stock No. 002994) should be 99.6% C57BL/6-like at all loci not linked to the Bax gene. At the present time The Jackson Laboratory's colony of C57BL/6J-Baxtm1Sjkmice are stillsegregating at the Tyr locus and retain the p allele from the 129 ES cell line. Thus, matings of Bax heterozygous (+/-) mice may produce progeny of varying coat color including black, white, chinchilla, light chinchilla, or grey, with all but the black mice having pink eyes. Because the potential for crossover between the p and Bax loci is small (roughly 5%), progeny that are grey in color and have pink eyes most likely are homozygous for the targeted Bax gene (-/-). However, we strongly recommend that you confirm the genotype of the mice prior to using them for research purposes. The Jackson Laboratory is continuing to backcross the Bax targeted mutation to C57BL/6J to try to eliminate the recessive 129 alleles of Tyr and p. However, in an effort to make these mice available to the research community as soon as possible we will begin distribution of the current colony. To summarize, the C57BL/6J-Baxtm1Sjkmice (Stock No. 002994) have been backcrossed 8 generations to the C57BL/6J inbred strain. However, because of coat color genes linked to the Bax mutation breeder pairs supplied by The Jackson Laboratory may produce progeny of varying coat color.
A Bax targeting vector substituted PGK-Neo for exons 2 through 5, deleting BH1 and BH2 and the capacity for a functional protein.
|Allele Name||targeted mutation 1, Stanley J Korsmeyer|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Bax1; Bax-|
|Gene Symbol and Name||Bax, BCL2-associated X protein|
|Strain of Origin||129X1/SvJ|
|Molecular Note||A neomycin selection cassette replaced exons 2 through 4 and part of exon 5, which encode sequences that correspond to the two BCL2 homology domains BH1 and BH2. Immunoblots did not detect the endcoded protein in tissue lysates derived from homozygous mice.|
|Mutations Made By|| |
Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute
The strain is maintained through heterozygous matings. BAX-deficient males are infertile and females are extremely poor breeders. Expected coat color from breeding:Black,Beige,Albino,Gray
When using the Bax<sup>-</sup> mouse strain in a publication, please cite the originating article(s) and include JAX stock #002994 in your Materials and Methods section.
|Heterozygous or Wild-type for Bax<tm1Sjk>|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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