B6.129-Ascl1^tm1And/J

Stock number: 002991
Product name: Mash-1^Δ
Research areas: Developmental Biology Research, Neurobiology Research

Description

These Ascl1 (formerly Mash-1) knock-out mice exhibit loss of sympathetic ganglia and olefactory epithelium. They are suitable for use in applications related to the study of nervous system development.

Detailed description

Mice homozygous for the Ascl1^tm1And targeted mutation die within 24 hours after birth. There are no gross external defects; However, mutant mice do have extensive loss of sympathetic ganglia and olefactory epithelium.

Allele

Symbol: Ascl1^tm1And
Name: targeted mutation 1, David J Anderson
MGI accession ID: MGI:1857470
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ascl1
Marker name: achaete-scute family bHLH transcription factor 1
Chromosome: 10
Strain of origin: 129/Sv
Molecular note: A PGK-neomycin resistance cassette replaced the entire coding region, 0.6 kb of sequence 5' of the translation initiation codon, and 0.2 kb of sequence 3' of the translation termination codon.
General note: ES cell line = D3 (129S2/SvPas) or R1 (129S1/Sv x 129X1/SvJ)F1. The authors state that most phenotypic analysis was done on R1-derived mice.