nNos KO mice may useful when studying structure and function of neurons and their synaptic connections in the brain and periphery.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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000664 C57BL/6J |
N11p+N3F11
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Nos1 | nitric oxide synthase 1, neuronal |
The mice of this strain have been backcrossed more than 10 backcrosses to C57BL/6J, making this strain more effective for studies of the Nos1 KO than the incipient stock (#002633).
The nitric oxide signaling molecule produced by the neuronal Nos1 gene is widely expressed, affecting diverse systems. The knockout of this gene has been shown to affect such normal functions as cognition and behavior, long-term memory, cortical development, colonic motility, skeletal muscle contractility, ovarian cycle regulation, cardiac/cardiovascular function, and certain immune system responses. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain.
Heterozygous mice are viable and fertile, while some lethality is seen in homozygotes due to stomach hypertrophy. The first publication describing this knock-out reported that mutants have enlarged stomachs with hypertrophy of the pyloric sphincter and the circular muscle layer (Huang et al., 1993). Male homozygotes of this strain were initially reported to be more aggressive than control mice, but this has been since attributed to heterogeneity of the stock before it was sufficiently backcrossed to the C57BL/6J genetic background. The C57BL/6J-congenic strain at The Jackson Laboratory (Stock No. 002986) shows normal behavior in this regard.
The Jackson Laboratory preferred mating scheme for Stock No. 002986 is breeding heterozygous mice together, or by breeding heterozygous females to homozygous males. Breeding heterozygous mice together has resulted in variable Mendelian ratios of homozygous pups (e.g., ~10% in September 2019 but ~24% in September 2020). Of note, we attempted breeding homozygotes together and found they generated offspring at a much slower rate.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to replace exon 1 of the nitric oxide synthase 1, neuronal (Nos1) gene with a neomycin cassette. This exon encodes the initiation site and amino acids 1 - 159 of the protein. The targeting vector was electroporated into 129S4/SvJae
-derived Jl ES cells that were injected into C57BL/6 blastocysts. Injected blastocysts were implanted in (C57BL/6 x DBA/2)Fl mice. Chimeric progeny were backcrossed to C57BL/6 mice. Some mice were further backcrossed to C57BL/6J mice (Stock No. 000664) for more than 10 generations to produce this Stock No. 002986.
Allele Name | targeted mutation 1, Paul L Huang |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | alphaNOS1; Kn; N2KO; nNOS-; nNOS KO; nNOS-; NOSdelta |
Gene Symbol and Name | Nos1, nitric oxide synthase 1, neuronal |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 5 |
Molecular Note | A neomycin cassette replaced exon 1. This exon encodes the initiation site and amino acids 1 - 159 of the protein. Northern blots of brain homogenates from homozygous mutant mice showed no detectable mRNA. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. |
Mutations Made By | Dr. Paul Huang, Mass General Hospital, Harvard Med Sch |
The Jackson Laboratory preferred mating scheme for Stock No. 002986 is breeding heterozygous mice together, or by breeding heterozygous females to homozygous males. Breeding heterozygous mice together has resulted in variable Mendelian ratios of homozygous pups (e.g., ~10% in September 2019 but ~24% in September 2020). Of note, we attempted breeding homozygotes together and found they generated offspring at a much slower rate.
Of note, we attempted breeding homozygotes together and found they generated offspring at a much slower rate.
Some lethality related to stomach hypertrophy observed in homozygotes.
When using the nNOS KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002986 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous or Wild-type for Nos1<tm1Plh> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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