Mouse Strain Datasheet - 002986
Mouse Strain Datasheet - 002986
nNos KO mice may useful when studying structure and function of neurons and their synaptic connections in the brain and periphery.
IMR Colony, The Jackson Laboratory
| Genetic Background | Generation |
|---|---|
|
000664 C57BL/6J |
N11+N3F9
|
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Null/Knockout) | Nos1 | nitric oxide synthase 1, neuronal |
The mice of this strain have been backcrossed more than 10 backcrosses to C57BL/6J, making this strain more effective for studies of the Nos1 KO than the incipient stock (#002633).
The nitric oxide signaling molecule produced by the neuronal Nos1 gene is widely expressed, affecting diverse systems. The knockout of this gene has been shown to affect such normal functions as cognition and behavior, long-term memory, cortical development, colonic motility, skeletal muscle contractility, ovarian cycle regulation, cardiac/cardiovascular function, and certain immune system responses. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. Heterozygous mice are viable and fertile, while some lethality is seen in homozygotes due to stomach hypertrophy. The first publication describing this knock-out reported that mutants have enlarged stomachs with hypertrophy of the pyloric sphincter and the circular muscle layer (Huang et al., 1993). Male homozygotes of this strain were initially reported to be more aggressive than control mice, but this has been since attributed to heterogeneity of the stock before it was sufficiently backcrossed to the C57BL/6J genetic background. Mice of this congenic strain in the Jackson Laboratory colony show normal behavior in this regard.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to replace exon 1 of the nitric oxide synthase 1, neuronal (Nos1) gene with a neomycin cassette. This exon encodes the initiation site and amino acids 1 - 159 of the protein. The targeting vector was electroporated into 129S4/SvJae
-derived Jl ES cells that were injected into C57BL/6 blastocysts. Injected blastocysts were implanted in (C57BL/6 x DBA/2)Fl mice. Chimeric progeny were backcrossed to C57BL/6 mice. Some mice were further backcrossed to C57BL/6J mice (Stock No. 000664) for more than 10 generations to produce this Stock No. 002986.
| Allele Name | targeted mutation 1, Paul L Huang |
|---|---|
| Allele Type | Targeted (Null/Knockout) |
| Allele Synonym(s) | targeted mutation 1, Paul L Huang; Nos1tm1Plh |
| Gene Symbol and Name | Nos1, nitric oxide synthase 1, neuronal |
| Gene Synonym(s) | Nos-1; NC-NOS; NOS; Nos-1; nNOS; NO; bNOS; 2310005C01Rik; N-NOS; nitric oxide synthase 1 (macrophage); 2310005C01Rik; RIKEN cDNA 2310005C01 gene; IHPS1 |
| Strain of Origin | 129S4/SvJae |
| Chromosome | 5 |
| Molecular Note | A neomycin cassette replaced exon 1. This exon encodes the initiation site and amino acids 1 - 159 of the protein. Northern blots of brain homogenates from homozygous mutant mice showed no detectable mRNA. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. |
| Mutations Made By | Dr. Paul Huang, Mass General Hospital, Harvard Med Sch |
This strain may be maintained by breeding heterozygous siblings due to some lethality related to stomach hypertrophy observed in homozygotes.
When using the nNOS KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002986 in your Materials and Methods section.
| Service | Genotype | Price |
|---|---|---|
| Heterozygous or Wild-type for Nos1<tm1Plh> |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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