Mice homozygous for the Il12rbtm1Jm targeted mutation are viable and fertile. Con A-activated splenocytes from homozygous mutant mice express only low affinity IL12-binding sites and fail to proliferate or produce interferon gamma in response to IL12. In addition, NK lytic activity in response to IL12 is defective. In vivo immune responses to endotoxin are also impaired.
This strain was made through homologous recombination in ES cells; neo replaces exons 1-3.
|Allele Name||targeted mutation 1, Jeanne Magram|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-12Rbeta1-; IL-12Rbeta1KO|
|Gene Symbol and Name||Il12rb1, interleukin 12 receptor, beta 1|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A neomycin resistance cassette replaced exons 1 to 3 of the gene, deleting the ATG translation initiation codon and causing a frame-shift in the translation reading frame.|
|Mutations Made By|| |
Dr. Jeanne Magram, Celsius Therapeutics
Homozygotes are viable and fertile. The expected coat color is black.
When using the IL-12Rβ1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002984 in your Materials and Methods section.