C57BL/6-Itgb7^tm1Cgn/J

Stock number: 002965
Product name: beta7^-
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for Itgb7^tm1Cgn show impaired formation of gut-associated lymphoid tissue (GALT). These mice may be useful for studies lymphocyte migration and homing in inflammatory and colitis related pathology.

Detailed description

Mice homozygous for the Itgb7^tm1Cgn targeted mutation have a marked hypoplasia of the gut-associated lymphoid tissue (GALT). This deficit is apparently due to the failure of Itgb7-deficient lymphocytes to arrest and adhere in the GALT. Beta7 integrin deficient mice also fail to mount a normal intestinal IgA response to ovalbumin and cholera toxin, and they show less graft-versus-host disease because of decreased homing of alloreactive T cells to intestine.

Development

The Itgb7^tm1Cgn targted mutation was made by Norbert Wagner in the laboratory of Dr. Klaus Rajewsky at the University of Cologne. The targeting construct was designed to replace part of exon 12 as well as exons 13 and 14 of the Itgb7 gene with a vector containing the neo resistance gene. The C57BL/6-derived B6III ES cell line was used.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Itgb7^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1861953
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Itgb7
Marker name: integrin beta 7
Chromosome: 15
Strain of origin: C57BL/6
Molecular note: A neomycin resistance cassette replaced part of exon 12 and all of exons 13 and 14, which encode the transmembrane and part of the extracellular domains.