Mice homozygous for Itgb7tm1Cgn show impaired formation of gut-associated lymphoid tissue (GALT). These mice may be useful for studies lymphocyte migration and homing in inflammatory and colitis related pathology.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Mice homozygous for the Itgb7tm1Cgn targeted mutation have a marked hypoplasia of the gut-associated lymphoid tissue (GALT). This deficit is apparently due to the failure of Itgb7-deficient lymphocytes to arrest and adhere in the GALT. Beta7 integrin deficient mice also fail to mount a normal intestinal IgA response to ovalbumin and cholera toxin, and they show less graft-versus-host disease because of decreased homing of alloreactive T cells to intestine.
The Itgb7tm1Cgn targted mutation was made by Norbert Wagner in the laboratory of Dr. Klaus Rajewsky at the University of Cologne. The targeting construct was designed to replace part of exon 12 as well as exons 13 and 14 of the Itgb7 gene with a vector containing the neo resistance gene. The C57BL/6-derived B6III ES cell line was used.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||beta7-; Itgbtm1Ctg|
|Gene Symbol and Name||Itgb7, integrin beta 7|
|Strain of Origin||C57BL/6J|
|Molecular Note||A neomycin resistance cassette replaced part of exon 12 and all of exons 13 and 14, which encode the transmembrane and part of the extracellular domains.|
|Mutations Made By|| |
Norbert Wagner, University of Cologne
When using the beta7- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002965 in your Materials and Methods section.