B6.129-Dll1^tm1Gos/J

Stock number: 002957
Product name: Dll1 KO
Research areas: Developmental Biology Research, Neurobiology Research

Description

These Dll1 knock-out mice exhibit defects in the segmentation of mesoderm in the developing embryo. They are suitable for use in applications related to the study of somite formation and patterning during embryogenesis.

Detailed description

Mouse embyros homozygous for the Dll1^tm1Gos targeted mutation establish a primary metameric pattern in the mesoderm. Cytodifferentiation appears normal, but the segments have no cranio-caudal polarity, and no epithelial somites form. Caudal sclerotome halves do not condense, and the pattern of spinal ganglia and nerves is perturbed, indicating loss of segment polarity. Myoblasts span segment borders, demonstrating that these borders are not maintained.

Development

The replacement vector was constructed by inserting a 4-kilobase ClaI/XhoI fragment, which encodes a part of the beta-galactosidase gene followed by the PGK-neo cassette, and a 2.6-kb XhoI/EcoRI fragment as the 3' homologous region of the replacement vector into pKS+.

Allele

Symbol: Dll1^tm1Gos
Name: targeted mutation 1, Achim Gossler
MGI accession ID: MGI:1861919
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Dll1
Marker name: delta like canonical Notch ligand 1
Chromosome: 17
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: In-frame insertion of a LacZ-PGK-neomycin resistance cassette replaced sequences that code for amino acids 2-116 of the Dll1 gene.