These Dll1 knock-out mice exhibit defects in the segmentation of mesoderm in the developing embryo. They are suitable for use in applications related to the study of somite formation and patterning during embryogenesis.
Dr. Achim Gossler, Medizinische Hochschule Hannover
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Dll1 | delta like canonical Notch ligand 1 |
Mouse embyros homozygous for the Dll1tm1Gos targeted mutation establish a primary metameric pattern in the mesoderm. Cytodifferentiation appears normal, but the segments have no cranio-caudal polarity, and no epithelial somites form. Caudal sclerotome halves do not condense, and the pattern of spinal ganglia and nerves is perturbed, indicating loss of segment polarity. Myoblasts span segment borders, demonstrating that these borders are not maintained.
The replacement vector was constructed by inserting a 4-kilobase ClaI/XhoI fragment, which encodes a part of the beta-galactosidase gene followed by the PGK-neo cassette, and a 2.6-kb XhoI/EcoRI fragment as the 3' homologous region of the replacement vector into pKS+.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, Achim Gossler |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Dll1-; Dll1lacZ |
Gene Symbol and Name | Dll1, delta like canonical Notch ligand 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 17 |
Molecular Note | In-frame insertion of a LacZ-PGK-neomycin resistance cassette replaced sequences that code for amino acids 2-116 of the Dll1 gene. |
Mutations Made By | Dr. Achim Gossler, Medizinische Hochschule Hannover |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes are not viable. The expected coat color from breeding is black.
When using the Dll1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002957 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Dll1<tm1Gos> |
Frozen Mouse Embryo | B6.129-Dll1<tm1Gos>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129-Dll1<tm1Gos>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129-Dll1<tm1Gos>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129-Dll1<tm1Gos>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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