These TGFα line 254 mice express a transgene containing the normal allele of the human transforming growth factor alpha gene (TGFα) under control of the mouse mammary tumor virus (MMTV) LTR and exhibit alveolar and sebaceous gland hyperplasia.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
This strain is homozygous for the retinal degeneration allele Pde6brd1.
Mice carrying the (MMTVTGFA)254Rjc transgene are viable and fertile, but lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgenic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females transgene expression was localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also revealed some stromal staining in the hyperplastic areas. Egfr mRNA expression was also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The B6D2-TgN(MMTVTGFA)254Rjc line is notable for sebaceous gland hyperplasia, which occurs in both male and female mice. The hyperplasia was evidenced as multiple raised, white plaques and nodules on the abdominal skin. No dysplasia or sebaceous cell carcinoma was observed. High expression of TGFa mRNA and TGFa protein was detected in the areas of sebaceous hyperplasia.
Expressed Gene | TGFA, transforming growth factor alpha, human |
---|---|
Site of Expression |
Allele Name | transgene insertion 254, Robert J Coffey |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(MMTVTGFA)254Rjc, transgene insertion 254, Robert J Coffey |
Gene Synonym(s) | |
Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV |
Expressed Gene | TGFA, transforming growth factor alpha, human |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | The transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced. |
Mutations Made By | Dr. Robert Coffey, Vanderbilt University Medical Center |
When maintaining a live colony, wildtype (non-carrier) females from the colony or FVB/NJ inbred females (Stock No. 001800) may be bred to transgene carrier males. The expected coat color from breeding is White Bellied Agouti, Albino.
When using the TGFα line 254 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002953 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrier for Tg(MMTVTGFA)254Rjc |
Frozen Mouse Embryo | FVB.Cg-Tg(MMTVTGFA)254Rjc/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Tg(MMTVTGFA)254Rjc/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Tg(MMTVTGFA)254Rjc/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | FVB.Cg-Tg(MMTVTGFA)254Rjc/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.