Mice homozygous mice for Tap1tm1Arp lack CD4-8+ T cells. They are defective in the stable assembly and intracellular transport of class I molecules, and show severely reduced levels of class I surface molecules. This phenotype is similar to human beings with TAP1 and TAP2 deficiencies.
IMR Colony, The Jackson Laboratory
Mice homozygous mice for the Tap1tm1Arp targeted mutation are viable and fertile. They are defective in the stable assembly and intracellular transport of class I molecules. They show severely reduced levels of class I surface molecules. They lack CD4-8+ T cells. This phenotype is similar to the mouse RMA-S cell line and human beings with TAP1 and TAP2 deficiencies. This strain should be housed under pathogen free conditions similar to the Prkdcscid/Prkdcscid mouse or any other immunodeficient strain.
The strain was developed in the laboratory of Dr. Philip Ashton-Rickardt, Imperial College London. A 7kb region of the wild-type locus (which made up 80% of the protein coding region) was replaced by a neomycin resistance cassette. The targeting construct was transfected into ES cells of the D3 line (derived from the strain 129/Sv) and correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric mice were bred with C57BL/6 mice and mice heterozygous for the mutation were interbred to homozygosity, then backcrossed to C57BL/6J for 10 generations before being maintained by inbreeding.
|Allele Name||targeted mutation 1, Philip Ashton-Rickardt|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Abcb2tm1Arp; TAP-; TAP-1-; TAP1-|
|Gene Symbol and Name||Tap1, transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A 7kb region of the wild-type locus (which made up 80% of the protein coding region) was replaced by a neomycin resistance cassette.|
|Mutations Made By|| |
Dr. Philip Ashton-Rickardt, Imperial College London
The Tap1 deficient strain is maintained by homozygous sibling matings. This strain should be housed under pathogen free conditions similar to the Prkdcscid/Prkdcscid mouse or any other immunodeficient strain. The expected coat color from breeding is Black.
When using the B6.129S2-Tap1tm1Arp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002944 in your Materials and Methods section.
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