Mice homozygous for the Kdrtm1Jrt targeted mutation (formerly called Flk1) die at ~E8.5-E9.5 due to defects in hematopoietic and endothelial development. Embryos lack blood islands at E7.5 and fail to form organized blood vessels. There is severe reduction in hematopoietic progenitor cells.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Kdr | kinase insert domain protein receptor |
Mice homozygous for the Kdrtm1Jrt targeted mutation (formerly called Flk1) die at ~E8.5-E9.5 due to defects in hematopoietic and endothelial development. Embryos lack blood islands at E7.5 and fail to form organized blood vessels. There is severe reduction in hematopoietic progenitor cells.
This strain was developed in the lab of Dr. Janet Rossant at the University of Toronto. The insertion vector contains lacZ and neo.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | The vascular endothelial lineage. |
Allele Name | targeted mutation 1, Janet Rossant |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | flk.lacZ; flk-1-; Flk1-; flk-1/lacZ; Flk1lacZ; Flk1lacZ; Flk1-lacZ neo-in; VEGFR-2-; Vegfr2lacZ |
Gene Symbol and Name | Kdr, kinase insert domain protein receptor |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | The vascular endothelial lineage. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 5 |
Molecular Note | Insertion of an in-frame lacZ gene and a floxed PGK-neomycin resistance cassette replaced the translated portion of the first coding exon and the proximal part of the next intron, and placed lacZ under the transcriptional control of the endogenous promoter. Neither RT-PCR using total RNA nor Western blot analysis using a carboxy terminal antibody detected gene expression in E8.5 homozygous mutant embryos. |
Mutations Made By | Dr. Janet Rossant, The Hospital for Sick Children |
When using the flk-1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002938 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Kdr<tm1Jrt> |
Frozen Mouse Embryo | B6.129-Kdrtm1Jrt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129-Kdrtm1Jrt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129-Kdrtm1Jrt/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129-Kdrtm1Jrt/J Frozen Embryos | $3373.50 |
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