B6.129-Kdr^tm1Jrt/J

Stock number: 002938
Product name: flk-1-
Research areas: Cancer Research, Cardiovascular Research, Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Detailed description

Mice homozygous for the Kdr^tm1Jrt targeted mutation (formerly called Flk1) die at ~E8.5-E9.5 due to defects in hematopoietic and endothelial development. Embryos lack blood islands at E7.5 and fail to form organized blood vessels. There is severe reduction in hematopoietic progenitor cells.

Development

This strain was developed in the lab of Dr. Janet Rossant at the University of Toronto. The insertion vector contains lacZ and neo.

Allele

Symbol: Kdr^tm1Jrt
Name: targeted mutation 1, Janet Rossant
MGI accession ID: MGI:1857168
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Synonyms: flk.lacZ; flk-1-; Flk1-; flk-1/lacZ; Flk1^lacZ; Flk1lacZ; Flk1-lacZ neo-in; VEGFR-2^-; Vegfr2^lacZ
Marker symbol: Kdr
Marker name: kinase insert domain protein receptor
Marker synonyms: CD309; FLK1; Flk-1; orv; vascular endothelial growth factor receptor- 2; VEGF receptor-2; VEGFR; VEGFR2; VEGFR-2
Chromosome: 5
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: The vascular endothelial lineage.
Molecular note: Insertion of an in-frame lacZ gene and a loxP site flanked neomycin resistance gene cassette replaced the translated portion of the first coding exon and the proximal part of the next intron, and placed lacZ under the transcriptional control of the endogenous promoter. Neither RT-PCR using total RNA nor Western blot analysis using a carboxy terminal antibody detected gene expression in E8.5 homozygous mutant embryos.