These int-1 mice express a transgene containing an MMTV LTR placed 5' to the Wnt1 (formerly int-1) gene in the opposite transcriptional orientation. Mammary glands of these mice are grossly hyperplastic.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mammary glands from virgin hemizygous females carrying the (Wnt1)1Hev transgene resemble hormonally-stimulated glands from pregnant mice. There was a marked increase in numbers of terminal branches and alveoli, producing a diffuse lobular hyperplasia. Parous transgenic females were unable to lactate. Hemizygous transgenic male glands, while less developed, also were hyperplastic. Adenocarcinomas developed between 3 and 7 months in females and more rarely in males. Occasional metastatic lesions were observed in females. Salivary adenocarcinomas were also occasionally observed in both males and females. Tumors arose stochastically, indicating additional events are required for neoplasia. Subsequent work with Wnt1 transgenic mice includes infection with MMTV to identify contributing oncogenes and mating to transgenic mice bearing other oncogenes.
The construct contained an MMTV LTR about 1 kb upstream from a Wnt1 genomic clone, with SV40 splice and polyadenylation sites added at the 3' end, and was microinjected into (C57BL/6 X SJL)F1 fertilized eggs. The strain was backcrossed to FVB/NJ for 11 generations.
Expressed Gene | Wnt1, wingless-type MMTV integration site family, member 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Harold E Varmus |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | line 303; MMTV-wnt; MMTV-Wnt1; MMTV-Wnt-1; Wnt-1 |
Gene Symbol and Name | Tg(Wnt1)1Hev, transgene insertion 1, Harold E Varmus |
Gene Synonym(s) | |
Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV |
Expressed Gene | Wnt1, wingless-type MMTV integration site family, member 1, mouse, laboratory |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
Molecular Note | Wnt1 is driven by the mouse mammary tumor virus (MMTV). The construct resembles alleles found in virus-induced tumors. |
Mutations Made By | Dr. Harold Varmus, Weill Cornell Medical College |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800).
The expected coat color from breeding is albino.
When using the FVB.Cg-Tg(Wnt1)1Hev/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002934 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous males and Non carrier females for Tg(Wnt1)1Hev |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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