In addition to the Tlr4Lps-d congenic interval from C3H/HeJ, this strain is also congenic for the wild type tyrosinase allele from C3H/HeJ on chromosome 7. This strain provides a tool for analysis of markers in the region and for examining functional effects of Lpsd on BALB/c, a strain susceptible to infection, neoplastic disease including the induction of plasmacytomas and other tumors.Read More +
In addition to the Tlr4Lps-d congenic interval from C3H/HeJ, this strain is also congenic for the wild type tyrosinase allele from C3H/HeJ on chromosome 7. This strain provides a tool for analysis of markers in the region and for examining functional effects of Lpsd on BALB/c, a strain susceptible to infection, neoplastic disease including the induction of plasmacytomas and other tumors.
The Lps-d mutation arose in C3H/HeJ sometime between 1960 and 1968. In 1977 a C3H/HeJ female, derived from the colony of David Sachs at NIH, was crossed with a BALB/cAnPt male and the Tlr4Lps-d allele was transferred onto the BALB/cAnPt background by continued backcrossing. At that time it was thought that Tlr4 and Tyrp1 were tightly linked so the agouti mice, those that would be carrying the C3H/HeJ-derived dominant B (wild-type) allele of Typr1 rather than being homozygous for the BALB/cAnPt-derived recessive b allele and Tyrc allele, were selected at each generation to continue the backcross. At N6 intercrossing made the strain homozygous for Tlr4Lps-d and Tyrp1B. Backcrossing to BALB/cAnPt was resumed from N6F22. When backcrossing reached N20, the strain was intercrossed to generate this strain homozygous for Tlr4Lps-d and Tyrp1B but not Tyrc. The congenic interval encompassing Tyrp1B and Tlr4Lps-d includes at least from D4Mit151 through D4Mit26. This strain was imported into The Jackson Laboratory from Dr.Stefanie Vogel at Uniformed Services University of the Health Sciences in 1997.
|Allele Name||defective lipopolysaccharide response|
|Allele Synonym(s)||Tlr4Lps-d; defective lipopolysaccharide response|
|Gene Symbol and Name||Tlr4, toll-like receptor 4|
|Gene Synonym(s)||ARMD10; Rasl2-8; Rasl2-8; CD284; lipopolysaccharide response; Lps; lipopolysaccharide response; Lps; TLR-4; RAS-like, family 2, locus 8; TOLL|
|Strain of Origin||C3H/HeJ|
|General Note||C3H/HeJ mice carry this allele. Various combinations of Lps-associated traits have been followed in crosses between C3H/HeJ and other C3H substrains, and the traits have in all cases segregated together (J:30692, J:5557, J:5593, J:5938). Some of the traits show dominance of the Tlr4lps-n allele; others, including Tlr4Lps-d, show codominance.
Genbank ID for this allele: AF095353
|Molecular Note||This allele corresponds to a mutation in the third exon of the gene. A C to A substitution at nucleotide position 2342 results in an amino acid substitution that replaces proline with histidine at position 712.|
The Tlr4Lps-d mutation arises from an A to C at substitution (Poltorak A et al. 1998. Science 282(5396):2085-8.[PMID: 9851930]) A single-strand conformation polymorphism (SSCP)-based assay for the Tlr
When using the C.C3-Tlr4Lps-d/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002930 in your Materials and Methods section.
|Homozygous for Tlr4<Lps-d>, 1 pair minimum|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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