These mice carry an ENU-induced mutation characterized by a lengthening of their circadian period.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Clock | circadian locomotor output cycles kaput |
Mice heterozygous for this Clockdelta19 mutation (also called Clockmut or Clockm1Jt) show a lengthening of their circadian period by about 1 hour. Homozygous mice show a lengthening of 4 hours followed by a loss of circadian rhythm. Homozygous mice are viable. Male homozygotes are fertile, but female homozygotes appear to have reduced fertility.
ENU-mutagenesis of male C57BL/6J mice created an A to T transversion at the third base position of the 5' splice donor site of intron 19. Exon 19 is missing from Clock mRNA, causing a 51 amino acid deletion within the glutamine-rich region of the protein's C terminus (amino acids 514-564). Mice with this Clockdelta19 mutation (also called Clockmut or Clockm1Jt) maintained on the C57BL/6J genetic background prior to sending to The Jackson Laboratory Repository.
Allele Name | Clock |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | Ck; Clockdelta19; Clockmut |
Gene Symbol and Name | Clock, circadian locomotor output cycles kaput |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 5 |
Molecular Note | ENU mutagenesis caused an A to T transversion at the third base position of the 5' splice donor site of intron 19. RT-PCR using primers for exon 15 and exon 21 detected shorter transcripts and no wild-type transcript in hypothalamus of homozygous mutant mice. Sequence analysis revealed that these shorter transcripts are missing exon 19. The authors predict the protein will be missing a 51 amino acid region within the carboxy terminus. |
Mutations Made By | Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr |
This strain is maintained by backcrossing heterozygous males to C57BL/6J females. Homozygous females don't breed well. This strain is currently on a C57BL/6J background (4/23/97) Expected coat color from breeding:Black
When using the Clock mouse strain in a publication, please cite the originating article(s) and include JAX stock #002923 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Clock<m1Jt> |
Frozen Mouse Embryo | C57BL/6J-Clockm1Jt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Clockm1Jt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Clockm1Jt/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Clockm1Jt/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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