Overview

Mice congenic for aryl hydrocarbon receptor (Ahr) alleles are useful in metabolic research and drug toxicity studies. Sensitivity to carcinogens often differs between the congenic and the recipient strain.

Donating Investigator

Dr. Alan Poland

Genetic overview

Genetic Background	Generation
000664 C57BL/6J

Ahr^d

Allele Type	Gene Symbol	Gene Name
Not Applicable	Ahr	aryl-hydrocarbon receptor

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Research Applications

Metabolism Research
Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

In this congenic strain, the DBA/2-derived d variant of the aryl hydrocarbon receptor gene (Ahr) was introgressed onto the C57BL/6N background. Ahr^d occurs naturally in DBA/2, AKR, 129, SWR, RF, and NZB strains. Mice congenic for Ahr alleles are useful in metabolic research and drug toxicity studies. Sensitivity to carcinogens often differs between the congenic and the recipient strain.

Development

The Ahr^d allele was backcrossed from DBA/2N onto C57BL/6N via a backcross-intercross breeding scheme by Dr. Daniel Nebert. At generation N13 this congenic was then transferred to Dr Alan Poland et al., who then backcrossed the Ahr^d allele onto C57BL/6J, again via a backcross-intercross breeding scheme, and homozygotes at or beyond generation N17 were sent to The Jackson Laboratory in 1997 and maintained by sibling intercross. The d allele found in DBA/2 differs from the b-1 allele found in C57BL by 10 nucleotides, five of which represent amino acid changes.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Poland A; Glover E. 1990. Characterization and strain distribution pattern of the murine Ah receptor specified by the Ahd and Ahb-3 alleles. Mol Pharmacol 38(3):306-12PubMed: 2169579 MGI: J:34840
Poland A; Palen D; Glover E. 1994. Analysis of the four alleles of the murine aryl hydrocarbon receptor. Mol Pharmacol 46(5):915-21PubMed: 7969080 MGI: J:22144

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Genetics

Ahr^d

Allele Symbol: Ahr^d

Allele Name	d variant
Allele Type	Not Applicable
Allele Synonym(s)	ah; Ah^d; Ah^k; Ahhⁿ; AhRd; in
Gene Symbol and Name	Ahr, aryl-hydrocarbon receptor
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	12
General Note	Strain of origin - this allele was found in DBA/2J, AKR/J, 129, SWR, RF, NZB strains
Molecular Note	This allele encodes a 104 kDa receptor that is stabilized by molybdate and has an affinity for ligand 10-100 fold lower than that of the receptor produced by the C57BL/6J allele. PCR sequencing of cDNA revealed ten nucleotide differences between the coding sequences of the DBA/2J and C57BL/6J receptors. Five of the ten differences would cause amino acid changes. One of these, an apparent T to C transition replaces the opal termination codon in the C57BL/6J allele with an arginine codon in the DBA/2J allele. This change would extend translation of the DBA/2J mRNA by 43 amino acids, accounting for the larger size of the peptide produced by this allele (104 kDa vs 95 kDa for the C57BL/6J allele). A second T to C transition changes a leucine codon in the C57BL/6J allele to a proline codon in the DBA/2J allele, and would likely change secondary structure of the peptide and thus ligand affinity.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ahr^d related

Metabolism Research
Research Tools
- Toxicology Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Ahr^d/Ahr^d
DBA/2J

homeostasis/metabolism phenotype

decreased physiological sensitivity to xenobiotic
- mice from mothers treated with TCDD exhibit a similar incidence of hydronephrosis as Ahr^b-1 homozygotes but at a reduced severity
- mice are more resistant to the teratogenic effects of TCDD than Ahr^b-1 homozygotes with only 30% of mice exhibiting altered palate development compared to 100% of Ahr^b-1 homozygotes
- mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

decreased sensitivity to xenobiotic induced morbidity/mortality
- mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

mortality/aging

decreased sensitivity to xenobiotic induced morbidity/mortality
- mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

Genotype: Ahr^d/Ahr^d
involves: 129S1/SvImJ * C57BL/6J

homeostasis/metabolism phenotype

increased incidence of tumors by chemical induction
- offspring exposed to DBP in utero exhibit an increased susceptibility to developing lymphomas
- offspring exposed to DBP in utero exhibit increased rates of lung cancer (though lower than in offspring of Ahr^d/Ahr^d dams) and liver tumors in male mice

increased sensitivity to xenobiotic induced morbidity/mortality
- mice are sensitive to in utero exposure to DBP and at three months of age develop difficulty breathing, anemia, hypoxia, large thoracic masses, enlarged spleens, livers and lymph nodes, and must be euthanized
- Normal - however, maternal genotype of Ahr^d/Ahr^d improves survival of offspring exposed to DBP in utero while maternal dietary exposure to I3C increased survival of offspring exposed to DBP in utero irregardless of offspring or dam genotype

increased physiological sensitivity to xenobiotic
- Normal - however, maternal genotype of Ahr^d/Ahr^d improves survival of offspring exposed to DBP in utero while maternal dietary exposure to I3C increased survival of offspring exposed to DBP in utero irregardless of offspring or dam genotype
- mice are sensitive to in utero exposure to DBP and at three months of age develop difficulty breathing, anemia, hypoxia, large thoracic masses, enlarged spleens, livers and lymph nodes, and must be euthanized

neoplasm

increased incidence of tumors by chemical induction
- offspring exposed to DBP in utero exhibit increased rates of lung cancer (though lower than in offspring of Ahr^d/Ahr^d dams) and liver tumors in male mice
- offspring exposed to DBP in utero exhibit an increased susceptibility to developing lymphomas

mortality/aging

increased sensitivity to xenobiotic induced morbidity/mortality
- Normal - however, maternal genotype of Ahr^d/Ahr^d improves survival of offspring exposed to DBP in utero while maternal dietary exposure to I3C increased survival of offspring exposed to DBP in utero irregardless of offspring or dam genotype
- mice are sensitive to in utero exposure to DBP and at three months of age develop difficulty breathing, anemia, hypoxia, large thoracic masses, enlarged spleens, livers and lymph nodes, and must be euthanized

Genotype: Ahr^d/Ahr^d
AKR

homeostasis/metabolism phenotype

decreased physiological sensitivity to xenobiotic
- mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

decreased sensitivity to xenobiotic induced morbidity/mortality
- mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

mortality/aging

decreased sensitivity to xenobiotic induced morbidity/mortality
- mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

The following phenotype relates to a compound genotype created using this strain

Genotype: Ahr^d/Ahr^d
B6.D2-Ahr

homeostasis/metabolism phenotype

decreased sensitivity to xenobiotic induced morbidity/mortality
- unlike C57BL/6-Ahr^b-1 neonates, mice are not susceptible to lethality induced by in utero exposure to cHBB

decreased physiological sensitivity to xenobiotic
- following exposure to cHBB in utero, neonates exhibit a lesser increase in volume density of hemopoietic islands in the liver compared to in C57BL/6-Ahr^b-1 neonates

mortality/aging

decreased sensitivity to xenobiotic induced morbidity/mortality
- unlike C57BL/6-Ahr^b-1 neonates, mice are not susceptible to lethality induced by in utero exposure to cHBB

References

Poland A; Glover E. 1990. Characterization and strain distribution pattern of the murine Ah receptor specified by the Ahd and Ahb-3 alleles. Mol Pharmacol 38(3):306-12PubMed: 2169579 MGI: J:34840
Poland A; Palen D; Glover E. 1994. Analysis of the four alleles of the murine aryl hydrocarbon receptor. Mol Pharmacol 46(5):915-21PubMed: 7969080 MGI: J:22144

Additional - Ahr^d related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Sanger sequencing:Ahr
- Genotyping resources and troubleshooting
Appearance
- black
  Related Genotype: a/a
Citation
When using the B6.D2N-Ahr^d/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002921 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous for Ahr<d>

Related Products and Services

Frozen Mouse Embryo	B6.D2N-Ahr<d>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.D2N-Ahr<d>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.D2N-Ahr<d>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.D2N-Ahr<d>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Ahrd

Allele Symbol: Ahrd

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ahrd related

Mammalian Phenotype Terms by Genotype

Genotype: Ahrd/AhrdDBA/2J

homeostasis/metabolism phenotype

mortality/aging

Genotype: Ahrd/Ahrdinvolves: 129S1/SvImJ * C57BL/6J

homeostasis/metabolism phenotype

neoplasm

mortality/aging

Genotype: Ahrd/AhrdAKR

homeostasis/metabolism phenotype

mortality/aging

Genotype: Ahrd/AhrdB6.D2-Ahr

homeostasis/metabolism phenotype

mortality/aging

References

Additional - Ahrd related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Ahr^d

Allele Symbol: Ahr^d

Genotype: Ahr^d related

Genotype: Ahr^d/Ahr^d
DBA/2J

Genotype: Ahr^d/Ahr^d
involves: 129S1/SvImJ * C57BL/6J

Genotype: Ahr^d/Ahr^d
AKR

Genotype: Ahr^d/Ahr^d
B6.D2-Ahr

Additional - Ahr^d related