These Dsg3 knock-out mice exhibit loss of cell adhesion with histology showing suprabasilar acantholysis most prominently on oral and vaginal mucous membranes.
Dr. John R. Stanley, U of Pennsylvania School of Medicine
Mice homozygous for Dsg3tm1Stan targeted mutation show loss of cell adhesion, resulting in a phenotype that resembles pemphigus vulgaris. By 15-20 days of age, homozygotes are smaller than unaffected littermates, perhaps due to erosions in the mouth (resulting in decreased food intake). Older mice show crusting lesions in areas of trauma. Histology shows suprabasilar acantholysis (i.e. loss of cell-cell adhesion in the basal and immediate suprabasal area). This is most prominent on oral and vaginal mucous membranes, but is also seen on the skin and eyelids. The mice start to lose hair at 3-4 weeks. The runting and hair loss phenotype are identical to that of a mouse mutant, balding bal. Allele testing indicated that bal is co-allelic with the targeted mutation.
|Allele Name||targeted mutation 1, John R Stanley|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Dsg3, desmoglein 3|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Replacement of part of the first exon, including the translation initiation site, with a neomycin cassette. Dsg3 RNA and protein were not detected in mice homozygous for this targeted mutation.|
|Mutations Made By|| |
Dr. John Stanley, U of Pennsylvania School of Medicine
Homozygous mice are poor breeders. Heterozygous females can be bred to homozygous males.
When using the DSG3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002911 in your Materials and Methods section.