B6;129X1-Dsg3^tm1Stan/J

Stock number: 002911
Product name: DSG3-
Research areas: Dermatology Research, Immunology, Inflammation and Autoimmunity Research, Mouse/Human Gene Homologs

Description

These Dsg3 knock-out mice exhibit loss of cell adhesion with histology showing suprabasilar acantholysis most prominently on oral and vaginal mucous membranes.

Detailed description

Mice homozygous for Dsg3^tm1Stan targeted mutation show loss of cell adhesion, resulting in a phenotype that resembles pemphigus vulgaris. By 15-20 days of age, homozygotes are smaller than unaffected littermates, perhaps due to erosions in the mouth (resulting in decreased food intake). Older mice show crusting lesions in areas of trauma. Histology shows suprabasilar acantholysis (i.e. loss of cell-cell adhesion in the basal and immediate suprabasal area). This is most prominent on oral and vaginal mucous membranes, but is also seen on the skin and eyelids. The mice start to lose hair at 3-4 weeks. The runting and hair loss phenotype are identical to that of a mouse mutant, balding bal. Allele testing indicated that bal is co-allelic with the targeted mutation.

Allele

Symbol: Dsg3^tm1Stan
Name: targeted mutation 1, John R Stanley
MGI accession ID: MGI:1857388
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Dsg3
Marker name: desmoglein 3
Chromosome: 18
Strain of origin: 129X1/SvJ
Molecular note: Replacement of part of the first exon, including the translation initiation site, with a neomycin cassette. Dsg3 RNA and protein were not detected in mice homozygous for this targeted mutation.