These Cyp2e1 knock-out mice show no obvious phenotypic abnormalities, but have elevated levels of the liver enzymes aspartate aminotransferase and alanine aminotransferase at high doses of acetaminophen. They may be suitable for use in applications related to the study of alcoholic liver disease.
Frank J Gonzalez, National Institutes of Health (NIH/NCI)
Exon 2 of the Cyp2e1 gene was replaced using a vector containing the neo resistance gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were crossed to 129S6/SvEvTac. The colony was maintained on a 129 background.
|Allele Name||targeted mutation 1, Frank J Gonzalez|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Cyp2e1, cytochrome P450, family 2, subfamily e, polypeptide 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exon 2 was replaced with a neomycin resistance cassette. Northern blot analysis demonstrated that the normal transcript was not present in liver of homozygous mice. Western blot analysis confirmed the absence of the protein in liver extracts derived from homozygous mice.|
|Mutations Made By|| |
Frank Gonzalez, National Institutes of Health (NIH/NCI)
This strain is maintained by mating homozygous siblings. The expected coat color from breeding is white-bellied agouti.
When using the Cyp2e1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002910 in your Materials and Methods section.