129/Sv-Cyp2e1^tm1Gonz/J

Stock number: 002910
Product name: Cyp2e1-
Research areas: Metabolism Research

Description

These Cyp2e1 knock-out mice show no obvious phenotypic abnormalities, but have elevated levels of the liver enzymes aspartate aminotransferase and alanine aminotransferase at high doses of acetaminophen. They may be suitable for use in applications related to the study of alcoholic liver disease.

Development

Exon 2 of the Cyp2e1 gene was replaced using a vector containing the neo resistance gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were crossed to 129S6/SvEvTac. The colony was maintained on a 129 background.

Allele

Symbol: Cyp2e1^tm1Gonz
Name: targeted mutation 1, Frank J Gonzalez
MGI accession ID: MGI:1857483
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cyp2e1
Marker name: cytochrome P450, family 2, subfamily e, polypeptide 1
Chromosome: 7
Strain of origin: 129S4/SvJae
Molecular note: Exon 2 was replaced with a neomycin resistance cassette. Northern blot analysis demonstrated that the normal transcript was not present in liver of homozygous mice. Western blot analysis confirmed the absence of the protein in liver extracts derived from homozygous mice.