129-Cyp1a2^tm1Gonz/J

Stock number: 002909
Product name: CYP1A2-
Research areas: Developmental Biology Research, Internal/Organ Research

Description

These Cyp1a2 knock-out mice exhibit perinatal death with impaired respiratory function. They are suitable for use in applications related to the study of neonatal respiratory distress syndrome.

Detailed description

Most homozygous 129/Sv-Cyp1a2^tm1Gonz mice die perinatally with impaired respiratory function due to lung immaturity. Amniotic fluid is not properly evacuated from the lungs at birth, and the alveoli do not fill with air. Lungs of homozygous mutant mice show lower levels of surfactant-associated protein (SAP) at birth than do wild type littermates. The penetrance of this phenotype is incomplete, as approximately 30% of homozygous mutants do survive to adulthood. Surviving animals, although they lack CYP1A2 protein, appear phenotypically normal and can reproduce.

Development

Exon 2 of the Cyp1a2 gene was disrupted using an insertion vector containing the neo resistance gene. The strain was developed on a 129/Sv background and they are currently at N10 on this background.

Allele

Symbol: Cyp1a2^tm1Gonz
Name: targeted mutation 1, Frank J Gonzalez
MGI accession ID: MGI:1861917
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cyp1a2
Marker name: cytochrome P450, family 1, subfamily a, polypeptide 2
Chromosome: 9
Strain of origin: 129S4/SvJae
Molecular note: A neomycin cassette was inserted into exon 2, which is the first coding exon. Western blot analysis on liver microsomes derived from adult homozygous mice demonstrated that no detectable protein was encoded by this allele.