These Soat1 knock-out mice exhibit tissue specific decreases in cholesterol esterification. They are suitable for use in applications related to the study of atherosclerosis.
Robert V Farese, Jr., Harvard University School of Public Health
Mice homozygous for the Soat1tm1 targeted mutation display tissue specific decreases in cholesterol esterification. Fibroblasts and adrenal membranes of homozygous mutant mice show diminished cholesterol esterification and markedly reduced cholesterol ester levels in adrenal glands and peritoneal macrophages. However, the livers of SOAT1-deficient mice contained substantial amounts of cholesterol esters and exhibited no reduction in cholesterol esterification activity. These findings support the presence of more than one form of esterification enzyme in mammals. A second sterol O-acyltransferase gene (Soat2), expressed in the liver and intestine, has subsequently been identified in mice and humans.
|Allele Name||targeted mutation 1, Bob Farese|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Acact-; Acat-1-|
|Gene Symbol and Name||Soat1, sterol O-acyltransferase 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Insertion of a neomycin cassette into a 5' early exon (amino acids 50-100). Reduced levels of a truncated mRNA transcript were detected by Northern blot on RNA from preputial glands. No protein was detected by immunoblot analysis in homogenates from preputial glands, ovaries and adrenals.|
|Mutations Made By|| |
Robert Farese, Jr., Harvard University School of Public Health
This strain is maintained by breeding homozygous siblings. Expected coat color from breeding:Black, White Bellied Agouti
When using the Acact- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002896 in your Materials and Methods section.