| Allele Name | transgene insertion 1, Werner Muller |
| Allele Type | Transgenic (Recombinase-expressing) |
| Allele Synonym(s) | bal1; Bal1 cre; balancer1; Nestin-Cre; SLNE4c; Tg(balancer1)2Cgn; TgN(balancer1)1Cgn; TgN(balancer1)2Cgn |
| Gene Symbol and Name | Tg(Nes-cre)1Wme, transgene insertion 1, Werner Muller |
| Gene Synonym(s) | |
| Promoter | Nes, nestin, mouse, laboratory |
|---|
| Expressed Gene | cre, cre recombinase, bacteriophage P1 |
|---|
| Site of Expression | induces variable mosaicism/partial deletion of loxP-flanked alleles before embryonic day 10.5 and in all adult organs (including germline), producing mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest |
|---|
| Strain of Origin | (C57BL/6 x CBA)F2 |
| Chromosome | UN |
| General Note | Mice carrying this "balancer 1" transgene show less variability and instability in their generation of mosaics than do transgenic mice carrying the "balancer 2" transgene. |
| Molecular Note | The transgenic construct contained a nestin promoter followed by cre recombinase sequence adjoined to a nuclear localization signal. Intron 2 of the nestin gene was included upstream of a polyadenylation signal. While the endogenous nestin promoter is CNS-specific, Southern blot analysis showed the ubiquitous expression of cre in transgenic mice. Variable mosaicism/partial deletion of loxP-flanked alleles occurs before embryonic day 10.5, and it is detectable in all adult organs examined including germ line cells. |
| Mutations Made By | Ulrich Betz, Business Group Pharma/Research |
