These bal1 mice express a transgene containing Cre recombinase under the control of the rat nestin promoter.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the balancer1 line.
The transgene contains the gene for Cre recombinase under the control of the rat nestin promoter and second intron enhancer. The "SLNE4c" construct was used. B6CBAF2 embryos were injected. This strain was submitted on a mixed C57BL/6, CBA, BALB/c, 129 background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | induces variable mosaicism/partial deletion of loxP-flanked alleles before embryonic day 10.5 and in all adult organs (including germline), producing mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest |
Allele Name | transgene insertion 1, Werner Muller |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | bal1; Bal1 cre; balancer1; Nestin-Cre; SLNE4c; Tg(balancer1)2Cgn; TgN(balancer1)1Cgn; TgN(balancer1)2Cgn |
Gene Symbol and Name | Tg(Nes-cre)1Wme, transgene insertion 1, Werner Muller |
Gene Synonym(s) | |
Promoter | Nes, nestin, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | induces variable mosaicism/partial deletion of loxP-flanked alleles before embryonic day 10.5 and in all adult organs (including germline), producing mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | UN |
General Note | Mice carrying this "balancer 1" transgene show less variability and instability in their generation of mosaics than do transgenic mice carrying the "balancer 2" transgene. |
Molecular Note | The transgenic construct contained a nestin promoter followed by cre recombinase sequence adjoined to a nuclear localization signal. Intron 2 of the nestin gene was included upstream of a polyadenylation signal. While the endogenous nestin promoter is CNS-specific, Southern blot analysis showed the ubiquitous expression of cre in transgenic mice. Variable mosaicism/partial deletion of loxP-flanked alleles occurs before embryonic day 10.5, and it is detectable in all adult organs examined including germ line cells. |
Mutations Made By | Ulrich Betz, Business Group Pharma/Research |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony. The expected coat color from breeding is Black,White Bellied Agouti
When using the bal1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002858 in your Materials and Methods section.
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Carrier or non-carrier for Tg(Nes-cre)1Wme |
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