B6;129S4-Nfkbia^tm1Bal/J

Stock number: 002850
Product name: IκBα-
Research areas: Dermatology Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research

Detailed description

Mice homozygous for the Nfkbia^tm1Bal mutation are runted, and have granulocytosis as well as abnormalities in epithelial keratinization. Neonates die at around 8 days of age.

Development

The Nfkbia gene was disrupted using an in frame insertion vector containing lacZ. The 129-derived J1 ES cell line was used.

Allele

Symbol: Nfkbia^tm1Bal
Name: targeted mutation 1, David Baltimore
MGI accession ID: MGI:1858084
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Synonyms: IkappaBalpha^-
Marker symbol: Nfkbia
Marker name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
Marker synonyms: EDAID2; I(Kappa)B(alpha); IkappaBalpha; I-kappaBalpha; IKBA; MAD-3; Nfkbi; RL/IF-1
Chromosome: 12
Strain of origin: 129S4/SvJae
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: The lacZ gene was inserted in frame 13 nucleotides downstream of the translational start site followed by a PGK-neomycin gene in reverse orientation thus replacing the remainder of the gene. Absence of protein product was established by Western blot analysis.