Mice homozygous for the Nfkbiatm1Bal mutation are runted, and have granulocytosis as well as abnormalities in epithelial keratinization. Neonates die at around 8 days of age.
Dr. David Baltimore, California Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Nfkbia | nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha |
Mice homozygous for the Nfkbiatm1Bal mutation are runted, and have granulocytosis as well as abnormalities in epithelial keratinization. Neonates die at around 8 days of age.
The Nfkbia gene was disrupted using an in frame insertion vector containing lacZ. The 129-derived J1 ES cell line was used.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, David Baltimore |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | IkappaBalpha- |
Gene Symbol and Name | Nfkbia, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129S4/SvJae |
Chromosome | 12 |
Molecular Note | The lacZ gene was inserted in frame 13 nucleotides downstream of the translational start site followed by a PGK-neomycin gene in reverse orientation thus replacing the remainder of the gene. Absence of protein product was established by Western blot analysis. |
Mutations Made By | Dr. David Baltimore, California Institute of Technology |
Homozygous mice die ~8 days of age. When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony.
When using the IκBα- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002850 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Nfkbia<tm1Bal> |
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