Mice homozygous for Nfkb1tm1Bal exhibit defective B cell responses, defective responses to infection, and also defects in basal and specific antibody production.
Dr. David Baltimore, California Institute of Technology
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Nfkb1 | nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 |
Mice homozygous for the Nfkb1tm1Bal targeted mutation are viable and fertile. Homozygous mutant mice exhibit defective B cell responses, defective responses to infection, and also defects in basal and specific antibody production.
Exon 6 of the Nfkb1 gene was disrupted by insertion of a vector containing the neo resistance gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to crossed to C57BL/6J.
Allele Name | targeted mutation 1, David Baltimore |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | NF-kappaBtm1Bal; Nfkappab1-; NF-kappaB1 KO; NF-kappaB1-; NFkappaB10; Nfkb1-; p105-; p50-; p50KO |
Gene Symbol and Name | Nfkb1, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 3 |
General Note | Effect of reconstitution with Nfkb1tm1Bal homozygous hematopoietic cells on atherogenesis in atherosclerosis prone miceTo assess the role of NFKB1 in atherogenesis, mice homozygous for a mutation of the low density lipoprotein receptor (Ldlrtm1Her) were lethally irradiated and transplanted with bone marrow from Nfkb1tm1Bal homozygous mice and 4 weeks later placed on a high-fat diet for 10 weeks. Aortic root lesion area of mice with NFKB1-deficient hematopoietic cells was 41% smaller than in control mice. Whereas control lesions contained primarily large foam cells, lesions of mice reconstituted with NFKB1-deficient bone marrow contained large numbers of small, inflammatory cells and very few foam cells. 3-fold as many cells attached to the lesion cap in transplanted mice. Most cells in control lesions were macrophages, while in transplanted mice there was a preponderance of T and B lymphocytes. Macrophages induced to differentiate in culture from NFKB1-deficient bone marrow exhibited differences compared to control macrophages in the secretion patterns of several cytokines following lipopolysaccharide (LPS) stimulation. Whereas control macrophages expressed high levels of scavenger receptor class A (SR-A) in response to LPS, this response was greatly attenuated in mice with NFKB1-deficient hematopoietic systems; uptake of oxidized low density lipoprotein (oxLDL) was similarly diminished, although neither parameter differed between transplant and control macrophages in the absence of stimulation. J:87639 |
Molecular Note | Insertion of a PGK-neomycin resistance cassette into exon 6 disrupted the gene. Exon 6 encodes the p105 precursor of the p50 subunit of the encoded transcription factor. The authors predict that this allele produces a truncated polypeptide that cannot bind with DNA, or dimerize with itself or other kappaB binding motifs. |
Mutations Made By | Dr. David Baltimore, California Institute of Technology |
While maintaining a live colony, these mice are maintained by mating homozygote siblings.
When using the p50- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002849 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Nfkb1<tm1Bal> |
Frozen Mouse Embryo | B6;129P-Nfkb1<tm1Bal>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P-Nfkb1<tm1Bal>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P-Nfkb1<tm1Bal>/J | $3373.50 |
Frozen Mouse Embryo | B6;129P-Nfkb1<tm1Bal>/J | $3373.50 |
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