B6;129P2-Fcer1g^tm1Rav/J

Stock number: 002847
Product name: FcRg-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Fcer1g knock-out mice exhibit immune system defects.

Detailed description

Mice homozygous for the Fcer1g^tm1Rav targeted mutation are viable and fertile. Immune system defects include: inability to phagocytose Ab-coated particles despite normal binding, defective NK cell-mediated Ab-dependent cytotoxicity and mast cell-mediated allergic responses. T cell development is normal. Inflammatory response to immune complexes is attentuated (lack of Arthus response). Homozygotes are completely resistant to experimental immune thrombocytopenia induced by mouse anti-platelet antibodies.

Development

A targeting vector containing a NEO cassette was used to disrupt exon 2, which created a new stop codon 239 bp downstream of the integration site. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and maintained on the mixed B6;129P2 background.

Allele

Symbol: Fcer1g^tm1Rav
Name: targeted mutation 1, Jeffrey V Ravetch
MGI accession ID: MGI:1857165
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Fcer1g
Marker name: Fc receptor, IgE, high affinity I, gamma polypeptide
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin selection cassette was inserted into exon 2, creating a new stop codon 239 bp downstream of the integration site. RT-PCR analysis on RNA isolated from activated macrophages, mast cells and NK cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on lysates derived from activated macrophages, mast cells and NK cells of homozygous mice confirmed that no protein is encoded by this allele.