These TgSREBP-1a460 mice express a transgene containing the human sterol regulatory element binding transcription factor 1 (SREBF1) under control of the rat phosphoenolpyruvate carboxykinase (Pck1) promoter and develop a fatty liver.
Robert E Hammer, UT Southwestern Medical Center at Dallas
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
The NH2-terminal domain of sterol-regulatory element binding protein-1a (SREBP-1a) activates transcription of genes encoding enzymes of cholesterol and fatty acid biosynthesis in cultured cells. These transgenic mice overexpress a truncated version of human SREBP-1a that includes the NH2-terminal domain but lacks the membrane attachment site. Expression is driven by the rat phosphoenolpyruvate carboxykinase (Pck1, also called Pepck) promoter, which gives high level expression in liver. When placed on a low carbohydrate/high protein diet to induce the Pck1 promoter the transgenic mice develop progressive and massive enlargement of the liver due to the engorgement of hepatocytes with cholesterol and triglycerides. The mRNAs encoding 3-hydroxy-3-methylglutaryl CoA (HMG CoA) synthase, HMG CoA reductase, squalene synthase, acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase-1 are elevated, as is the LDL receptor mRNA. The rates of cholesterol and fatty acid synthesis in liver is elevated 5- and 25-fold, respectively. Plasma lipid levels are not elevated. The amount of white adipose tissue decreases progressively as the liver enlarges. (Shimano H, et al., J Clin Invest. 1996, 98:1575)
Expressed Gene | SREBF1, sterol regulatory element binding transcription factor 1, human |
---|---|
Site of Expression |
Allele Name | transgene insertion 7343, Robert E Hammer |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | S+; Tg(SREBF1)2Mbjg; TgSREBP-1a |
Gene Symbol and Name | Tg(SREBP1a)7343Reh, transgene insertion 7343, Robert E Hammer |
Gene Synonym(s) | |
Promoter | Pepck, Pck1-phosphoenolpyruvate carboxykinase, |
Expressed Gene | SREBF1, sterol regulatory element binding transcription factor 1, human |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | The transgene contains human sterol regulatory element binding transcription factor 1 (SREBF1) under control of the phosphoenolpyruvate carboxykinase (Pck1) promoter. Seven lines were created. |
Mutations Made By | Robert Hammer, UT Southwestern Medical Center at Dallas |
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to B6SJLF1/J mice (Stock No. 10012).
When using the TgSREBP-1a460 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002840 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(SREBP1a)7343Reh |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.