These Plaur knock-out mice exhibit no overt phenotypic abnormalities with no compromise in fertility, development, or hemostasis.
Dr. Thomas Bugge, NIH/NIDCR
Homozygous mice are born and survive to adulthood with no overt phenotypic abnormalities. No Plaur gene product (protein) is detected. In addition, activated peritoneal macrophages collected from homozygous mice fail to promote plasminogen activation in vitro. The loss of the receptor also results in a redistribution of uPA in some tissues but has no impact on pro-PLAU activation in the urogenital tract. Thus, in the absence of other challenging factors such as infection, injury, or other functional deficits, PLAUR deficiency does not compromise fertility, development, or hemostasis. These mice provide a means to test the proposed function of PLAU/PLAUR in wound repair, atherogenesis, and tumor cell invasion in vivo.
This mutation was generated by replacing exon 3 with a vector containing Hprt. The 129P2-derived E14TG2a ES cell line was used.
|Allele Name||targeted mutation 1, Jay L Degen|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Plaurtm1Jld; targeted mutation 1, Jay L Degen|
|Gene Symbol and Name||Plaur, plasminogen activator, urokinase receptor|
|Gene Synonym(s)||uPAR-3; CD87; URKR; urokinase-type plasminogen activator receptor; UPAR; uPAR-2; U-PAR; Plaur3; Par; uPAR; u-PAR; Cd87|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exon 3, encoding the second half of the amino-terminal uPA binding domain, was replaced by an HPRT minigene.|
Homozygotes are viable and fertile. The expected coat color is black.
When using the uPAR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002829 in your Materials and Methods section.
|Heterozygous or Wild-type for Plaur<tm1Jld>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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