C.129S2-Stat6^tm1Gru/J

Stock number: 002828
Product name: Stat6^-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for Stat6^tm1Gru show a striking defect in the generation of Th2 cells; lymphocytes fail to proliferate in response to IL-4. These mice may be useful for studies of colitis and other inflammatory diseases.

Detailed description

Mice homozygous for the Stat6^tm1Gru mutation show a striking defect in the generation of Th2 cells. Lymphocytes are unable to respond to IL4 as measured in a variety of in vitro and in vivo assays. Stat6-deficient B cells do not produce IgE following in vivo immunization with anti-IgD. Mesenteric lymph node cells from STAT6^-/- mice with colitis exhibited reduced secretion of IL-4, IL-5, IL-13, and IFN-gamma. They also show impaired Th2 differentiation and reduced airway response to allergen. With enhanced renal injury, Th1 and Th17 nephritogenic immune responses were increased in STAT6^-/- mice while production of IL-5, a key Th2-associated cytokine, was decreased significantly.

Allele

Symbol: Stat6^tm1Gru
Name: targeted mutation 1, Michael J Grusby
MGI accession ID: MGI:1888385
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Stat6
Marker name: signal transducer and activator of transcription 6
Chromosome: 10
Strain of origin: 129S2/SvPas
Molecular note: The region encoding the SH2 domain, which is essential for dimerization, was replaced by the insertion of a neomycin selection cassette. Immunoblot analysis of lysates from the spleens and thymi of homozygous mutant mice showed an absence of encoded protein.