Mice homozygous for Stat6tm1Gru show a striking defect in the generation of Th2 cells; lymphocytes fail to proliferate in response to IL-4. These mice may be useful for studies of colitis and other inflammatory diseases.
Dr. Michael J. Grusby, Harvard Medical School
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Stat6 | signal transducer and activator of transcription 6 |
Mice homozygous for the Stat6tm1Gru mutation show a striking defect in the generation of Th2 cells. Lymphocytes are unable to respond to IL4 as measured in a variety of in vitro and in vivo assays. Stat6-deficient B cells do not produce IgE following in vivo immunization with anti-IgD. Mesenteric lymph node cells from STAT6-/- mice with colitis exhibited reduced secretion of IL-4, IL-5, IL-13, and IFN-gamma. They also show impaired Th2 differentiation and reduced airway response to allergen. With enhanced renal injury, Th1 and Th17 nephritogenic immune responses were increased in STAT6-/- mice while production of IL-5, a key Th2-associated cytokine, was decreased significantly.
Allele Name | targeted mutation 1, Michael J Grusby |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | S6KO; Stat6- |
Gene Symbol and Name | Stat6, signal transducer and activator of transcription 6 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 10 |
Molecular Note | The region encoding the SH2 domain, which is essential for dimerization, was replaced by the insertion of a neomycin selection cassette. Immunoblot analysis of lysates from the spleens and thymi of homozygous mutant mice showed an absence of encoded protein. |
Mutations Made By | Dr. Michael Grusby, Harvard Medical School |
When using the Stat6- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002828 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Stat6<tm1Gru> |
Frozen Mouse Embryo | C.129S2-Stat6<tm1Gru>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129S2-Stat6<tm1Gru>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129S2-Stat6<tm1Gru>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C.129S2-Stat6<tm1Gru>/J Frozen Embryos | $3373.50 |
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