Cells from homozygous Tnfrsf1atm1Mak mutant mice lack expression of TNFRp55, and TNF signaling is largely abolished. These mice may be useful in studying the multiple biological activities of tumor necrosis factor.
Dr. Tak Mak, University Health Network/Un of TorontoRead More +
Mice homozygous for the Tnfrsf1atm1Mak targeted mutation (formerly Tnfr1tm1Mak) have normal thymocyte development, lymphocyte populations and clonal deletion of potentially self-reactive T cells. TNF signaling is largely abolished, with no TNF induction of NF-kB. Homozygous mutant mice are resistant to lethal dosages of either lipopolysaccharides or S. aureus enterotoxin but succumb to L. monocytogenes infection. TNFRSF1an may also protect against atherosclerotic lesion development in mice fed an atherogenic diet.
|Allele Name||targeted mutation 1, Tak Mak|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Pfeffer Tnfrsf1a-; TNF-R1-; TNF-alphaRI-; TNFR-; TNFR1-; TNFR1/p55-; TNFRp55-; Tnfrsf1atm1Mak; Tnfrsf1a-; p55-|
|Gene Symbol and Name||Tnfrsf1a, tumor necrosis factor receptor superfamily, member 1a|
|Gene Synonym(s)||CD120a; FPF; MS5; TBP1; TNF receptor alpha chain; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNF-alpha-R1; TNF-alphaR1; TNFAR; TNFR-1; TNFR1; TNFR1-d2; TNFR55; TNFR60; TNFRI; TNFRp55; TNFalpha-R1; Tnfr-2; Tnfr1; Tnfr1; p55; p55-R; p60; tumor necrosis factor receptor 1; tumor necrosis factor receptor 2|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin cassette was inserted at position 535 of the coding sequence. A radioligand binding assay demonstrated that no functional protein was present on the cell surface of splenocytes derived from homozygous mice.|
|Mutations Made By|| |
Dr. Tak Mak, University Health Network/Un of Toronto
When maintaining a live colony, homozygous mice may be bred together.
|Please inquire about possible genotypes.|
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