C3.Cg-Pde6b⁺ Krd/J

Stock number: 002802
Research areas: Internal/Organ Research, Mouse/Human Gene Homologs, Sensorineural Research

Description

This strain combines the semidominant transgene insertion, Krd with the wild type allele of Pde6b. Mice heterozygous for Krd have a high incidence of kidney defects including aplastic, hypoplastic, and cystic kidneys. Retinal defects include abnormal electroretinograms and a reduction of cell numbers that is most extreme in the inner cell and ganglion layers. Homozygosity results in early embryonic lethality.

Detailed description

This strain combines the semidominant transgene insertion, Krd with the wild type allele of Pde6b. Mice heterozygous for Krd have a high incidence of kidney defects including aplastic, hypoplastic, and cystic kidneys. Retinal defects include abnormal electroretinograms and a reduction of cell numbers that is most extreme in the inner cell and ganglion layers. Homozygosity results in early embryonic lethality.

Development

(C57BL/6J x C3H)F1 eggs were microinjected with an amylase/rat elastase/chloramphenicol acetyltransferase construct and the founders were bred to C57BL/6J, then to YBR (Johnson et al., 1993). This strain came to The Jackson Laboratory from Dr. Miriam Meisler at the University of Michigan in 1995 as STOCK Tg8052/C3H at N2 to C3H. At The Jackson Laboratory it was bred to C3HeB/FeJ, then one generation to BALB/cByJ. The Krd/+ F1 offspring were then mated with C3.BliA-Pde6b⁺ (001912) at each generation to produce the congenic C3.BLiA-Pde6b⁺ -Krd/+ (002802) that we currently offer. The X and Y chromosomes from C3.BliA-Pde6b⁺ (001912) were captured and fixed in 002802 within the first 3 backcross generations. Since 1995 the Krd/+ breeders were selected primarily by expression of the retinal degeneration phenotype. This strain passed through only approximately 2 generations per year. In 2009 sperm was cryopreserved from Pde6b^+/+ Krd/+ males. Standard cryorecovery is scheduled to use C3H/HeJ, rather than the less readily available C3Sn.BLiA-Pde6b⁺/DnJ so the cryorecovered mice will be segregating for Pde6b⁺ and Pde6b^rd1.

Allele

Symbol: Krd
Name: kidney and retinal defects
MGI accession ID: MGI:1861657
Generation method: Transgenic
Synonyms: Tg8052
Marker symbol: Krd
Marker name: kidney and retinal defects
Marker synonyms: Del(19)TgN8052Mm
Chromosome: 19
Strain of origin: Not Specified
Molecular note: The phenotype of this mouse has been attributed to a 7 cM transgene induced deletion, Del(19)TgN8052Mm, which includes the Pax2 and Pkd2l1. The transgene inserted into a LINE element in the distal region of Chromosome 19. The Scd1 and pale ear Hps1 genes are also deleted in Del(19)TgN8052Mm mice, along with several D19Mit markers.

Allele

Symbol: Pde6b⁺
Name: wild type
MGI accession ID: MGI:2430003
Generation method: Not Applicable
Marker symbol: Pde6b
Marker name: phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Marker synonyms: CSNB3; CSNBAD2; GMP-PDEbeta; nmf137; PDEB; r; rd; rd1; rd10; RP40
Chromosome: 5
Strain of origin: Not Applicable
Expressed genes: Pde6b,phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide,mouse, laboratory
Site of expression: Retina.