This strain combines the semidominant transgene insertion, Krd with the wild type allele of Pde6b. Mice heterozygous for Krd have a high incidence of kidney defects including aplastic, hypoplastic, and cystic kidneys. Retinal defects include abnormal electroretinograms and a reduction of cell numbers that is most extreme in the inner cell and ganglion layers. Homozygosity results in early embryonic lethality.
Read More +Genetic Background | Generation |
---|---|
+F21
|
Allele Type |
---|
Transgenic |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | Pde6b | phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide |
This strain carries a Chromosome 19 deletion that includes: Abcc2, Fgf8, Nkx2-3, Pax2, Scd1, Tlx1, Wnt8b, and Hps1ep. This congenic is homozygous for the wildtype Pde6b+ allele.
This strain combines the semidominant transgene insertion, Krd with the wild type allele of Pde6b. Mice heterozygous for Krd have a high incidence of kidney defects including aplastic, hypoplastic, and cystic kidneys. Retinal defects include abnormal electroretinograms and a reduction of cell numbers that is most extreme in the inner cell and ganglion layers. Homozygosity results in early embryonic lethality.
(C57BL/6J x C3H)F1 eggs were microinjected with an amylase/rat elastase/chloramphenicol acetyltransferase construct and the founders were bred to C57BL/6J, then to YBR (Johnson et al., 1993). This strain came to The Jackson Laboratory from Dr. Miriam Meisler at the University of Michigan in 1995 as STOCK Tg8052/C3H at N2 to C3H. At The Jackson Laboratory it was bred to C3HeB/FeJ, then one generation to BALB/cByJ. The Krd/+ F1 offspring were then mated with C3.BliA-Pde6b+ (001912) at each generation to produce the congenic C3.BLiA-Pde6b+ -Krd/+ (002802) that we currently offer. The X and Y chromosomes from C3.BliA-Pde6b+ (001912) were captured and fixed in 002802 within the first 3 backcross generations. Since 1995 the Krd/+ breeders were selected primarily by expression of the retinal degeneration phenotype. This strain has passed through only approximately 2 generations per year.
Expressed Gene | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide, mouse, laboratory |
---|---|
Site of Expression | Retina. |
Allele Name | kidney and retinal defects |
---|---|
Allele Type | Transgenic |
Allele Synonym(s) | Tg8052 |
Gene Symbol and Name | Krd, kidney and retinal defects |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 19 |
Molecular Note | The phenotype of this mouse has been attributed to a 7 cM transgene induced deletion, Del(19)TgN8052Mm, which includes the Pax2 and Pkd2l1. The transgene inserted into a LINE element in the distal region of Chromosome 19. The Scd1 and pale ear Hps1 genes are also deleted in Del(19)TgN8052Mm mice, along with several D19Mit markers. |
Allele Name | wild type |
---|---|
Allele Type | Not Applicable |
Allele Synonym(s) | |
Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide |
Gene Synonym(s) | |
Expressed Gene | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide, mouse, laboratory |
Site of Expression | Retina. |
Strain of Origin | Not Applicable |
Chromosome | 5 |
When using the C3.Cg-Pde6b+ Krd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002802 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Please inquire |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.