In these Notch1 deficient mice, somitogenesis is disturbed and there is a probable defect in chorio-allantoic connection. Homozygotes die at embryonic day 9.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The mutant was generated by deleting a large portion of the Notch1 gene using a positive/negative targeting vector. These mice have been backcrossed to C57BL/6J mice (Stock No. 000664) for at least 10 generations.
|Allele Name||targeted mutation 1, Ronald L Conlon|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||N1delta1; Notch-; Notchdelta1; Notch1-; Notch1tm1Con/J|
|Gene Symbol and Name||Notch1, notch 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Deletion of sequences encoding the EGF repeats 28 to 36, three Notch repeats, the transmembrane domain and CDC10/SWI6 repeats, and replacement with a neomycin gene.|
|Mutations Made By|| |
Dr. Ronald Conlon, Case Western Reserve Univ, School of Med
This strain is maintained by breeding heterozygous mice to normal wildtype siblings or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes die at embryonic day 9.
When using the B6.129-Notch1tm1Con/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002797 in your Materials and Methods section.