B6.129-Notch1^tm1Con/J

Stock number: 002797
Product name: Notch1 KO [N1δ1]
Research areas: Developmental Biology Research

Description

Somitogenesis is disturbed in these Notch1 deficient mice.

Detailed description

In these Notch1 deficient mice, somitogenesis is disturbed and there is a probable defect in chorio-allantoic connection. Homozygotes die at embryonic day 9.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The mutant was generated by deleting a large portion of the Notch1 gene using a positive/negative targeting vector. These mice have been backcrossed to C57BL/6J mice (Stock No. 000664) for at least 10 generations.

Allele

Symbol: Notch1^tm1Con
Name: targeted mutation 1, Ronald L Conlon
MGI accession ID: MGI:1857230
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: N1^delta1; Notch^-; Notch^delta1; Notch1^-; Notch1^tm1Con/J
Marker symbol: Notch1
Marker name: notch 1
Marker synonyms: 9930111A19Rik; AOS5; AOVD1; hN1; lin-12; Mis6; Motch A; N1; NOTCH; TAN1
Chromosome: 2
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Deletion of sequences encoding the EGF repeats 28 to 36, three Notch repeats, the transmembrane domain and CDC10/SWI6 repeats, and replacement with a neomycin gene.