C.129S4-Tfap2a^tm1Jae/J

Stock number: 002794
Product name: AP-2 -
Research areas: Cancer Research, Developmental Biology Research, Neurobiology Research

Description

These Tcfap2a knock-out mice exhibit embryonic lethality with multiple severe developmental defects of the face, skull, sensory organs, and cranial ganglia.

Detailed description

Mice homozygous for the Tcfap2a^tm1Jae mutation die around embryonic day 9.0-9.5 due to failure of cranial closure. These embryos also show multiple severe developmental defects of the face, skull, sensory organs, and cranial ganglia. There is increased apoptosis in the midbrain of day 9 embryos, coinciding with failure of cranial closure.

Development

This strain was developed in the laboratory of Dr. Rudolf Jaenisch at the Massachusetts Institute of Technology. Exon 5 of the Tcfap2a gene was replaced with a vector containing the neo resistance gene. The 129-derived J1 ES cell line was used. Mice have been backcrossed to BALB/c mice.

Allele

Symbol: Tfap2a^tm1Jae
Name: targeted mutation 1, Rudolf Jaenisch
MGI accession ID: MGI:1888387
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tfap2a
Marker name: transcription factor AP-2, alpha
Chromosome: 13
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance cassette replaced a genomic fragment containing exon 5, which encodes part of the DNA binding domain of the protein.