Poly(ADP-ribose) polymerase-1 knock-out mice may be useful in studying DNA repair, programmed cell death, aging, differentiation, proliferation and tumor transformation.
Zhao-Qi Wang, International Agency for Research/Cancer
Genetic Background | Generation |
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F?+F15N3F9
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Parp1 | poly (ADP-ribose) polymerase family, member 1 |
Mice that are homozygous for the poly(ADP-ribose) polymerase-1 null mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Although a shortened transcript is generated, no enzymatic activity is detected in tissues. Proliferation of homozygous fibroblasts and thymocytes is impaired following gamma-radiation in comparison to cells derived from wildtype mice. Older mice are susceptible to spontaneous development of skin disease. A significant portion of older mice (~30%) can be expected to exhibit epidermal hyperplasia. Null mice are also less susceptible to damage induced by the neurotoxin MPTP.
A targeting vector containing a neomycin resistance gene was used to disrupt exon 2. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were obtained and bred to 129S6/SvEv mice. This strain originated and is maintained on a 129S background.
Allele Name | targeted mutation 1, Zhao-Qi Wang |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ADPRT-; Adprt1tm1Wag; ARTD1-; PARP-; PARP-1-; PARS-; PKO |
Gene Symbol and Name | Parp1, poly (ADP-ribose) polymerase family, member 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | A promoterless neomycin gene replaced part of exon 2 and intron 2. The neomycin sequences were fused in-frame to the coding sequence of the gene. Northern blot analysis detected a truncated fusion transcript in homozygous mice. Enzyme activity assays demonstrated a lack of functional ADPRT1 protein in homozygous mice. |
Mutations Made By | Zhao-Qi Wang, International Agency for Research/Cancer |
When maintaining a live colony, homozygous mice may be bred together.
When using the ADPRT- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002779 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous for Parp1<tm1Zqw> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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